| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNRH2 |
| Uniprot No | O43555 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-120aa |
| 氨基酸序列 | QHWSHGWYPGGKRALSSAQDPQNALRPPGRALDTAAGSPVQTAHGLPSDALAPLDDSMPWEGRTTAQWSLHRKRHLARTLLTAAREPRPAPPSSNKV |
| 预测分子量 | 26.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNRH2重组蛋白的3篇参考文献概览(注：文献信息为模拟生成，实际研究中请参考真实数据库)：
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1. **文献名称**：*Recombinant Human GNRH2: Expression in E. coli and Functional Characterization*
**作者**：Chen L. et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组人源GNRH2蛋白。通过体外受体结合实验，证实其能够特异性激活GNRH受体，并诱导下游信号通路(如MAPK/ERK)的激活，为后续生殖激素机制研究提供工具。
2. **文献名称**：*Structural Analysis of GNRH2 and Its Evolutionary Divergence from GNRH1*
**作者**：Smith J.R., Kumar S.
**摘要**：通过X射线晶体学解析重组GNRH2蛋白的三维结构，发现其与GNRH1在结合口袋构象上的关键差异，解释了二者在不同物种中的功能分化，提示GNRH2可能在非生殖系统中发挥独特作用。
3. **文献名称**：*GNRH2 Recombinant Protein Suppresses Ovarian Cancer Cell Proliferation via Autocrine Pathways*
**作者**：Wang Y. et al.
**摘要**：体外实验表明，重组GNRH2蛋白可通过结合卵巢癌细胞表面受体，抑制EGFR磷酸化并诱导细胞凋亡，提示其作为潜在抗癌治疗分子的可能性。
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**提示**：实际研究中建议通过PubMed、Google Scholar等平台，以关键词“GNRH2 recombinant protein”、“GnRH-II expression”等检索最新文献，并优先选择高影响因子期刊的研究。
**Background of GnRH2 Recombinant Protein**
Gonadotropin-releasing hormone 2 (GnRH2), also known as chicken GnRH-II, is a highly conserved neuropeptide belonging to the GnRH family, which plays pivotal roles in regulating reproductive physiology. Unlike the classical GnRH1 (mammalian GnRH), GnRH2 is encoded by a separate gene and is evolutionarily conserved across vertebrates, suggesting its fundamental biological significance. Structurally, GnRH2 differs from GnRH1 by three amino acids, contributing to distinct receptor-binding affinities and functional specificities.
GnRH2 is widely expressed in both neural and non-neural tissues, including the brain, reproductive organs, immune cells, and cancer cells, indicating multifunctional roles beyond gonadotropin secretion. It regulates reproductive behaviors, energy balance, and immune responses, and has been implicated in cell proliferation, apoptosis, and migration in cancer models. In humans, GnRH2 may act through the GnRH1 receptor (GnRHR1) or a putative GnRH2-specific receptor, though its signaling mechanisms remain less defined compared to GnRH1.
Recombinant GnRH2 protein is synthesized using biotechnological platforms (e.g., *E. coli*, yeast, or mammalian systems) to produce bioactive peptides for research and therapeutic applications. Its production enables studies on receptor interactions, signaling pathways, and physiological effects in vitro and in vivo. In biomedicine, GnRH2-based therapies are explored for treating reproductive disorders, hormone-dependent cancers, and inflammatory diseases, leveraging its potential to modulate hormone secretion or directly target tumor cells.
Despite its promise, challenges persist in understanding GnRH2’s precise physiological roles and optimizing receptor-specific analogs. Research on recombinant GnRH2 continues to unravel its therapeutic potential and evolutionary significance in vertebrate reproduction and homeostasis.
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