| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNAT2 |
| Uniprot No | P19087 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-354aa |
| 氨基酸序列 | GSGASAEDK ELAKRSKELE KKLQEDADKE AKTVKLLLLG AGESGKSTIV KQMKIIHQDG YSPEECLEFK AIIYGNVLQS ILAIIRAMTT LGIDYAEPSC ADDGRQLNNL ADSIEEGTMP PELVEVIRRL WKDGGVQACF ERAAEYQLND SASYYLNQLE RITDPEYLPS EQDVLRSRVK TTGIIETKFS VKDLNFRMFD VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDDEV NRMHESLHLF NSICNHKFFA ATSIVLFLNK KDLFEEKIKK VHLSICFPEY DGNNSYDDAG NYIKSQFLDL NMRKDVKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD CGLF |
| 预测分子量 | 40,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNAT2重组蛋白的3篇代表性文献示例(注：部分信息为模拟简化版，实际文献需通过学术数据库查询确认)：
---
1. **文献名称**：*Expression and purification of recombinant GNAT2 for functional analysis of cone phototransduction*
**作者**：Smith J. et al.
**摘要**：研究报道了在大肠杆菌系统中高效表达人源GNAT2重组蛋白的优化方法，并通过亲和层析纯化获得高纯度蛋白。实验验证了重组GNAT2与视锥细胞视蛋白的GTPase活性相互作用，为体外研究色觉信号传导机制提供了工具。
---
2. **文献名称**：*Structural insights into GNAT2 mutations associated with achromatopsia*
**作者**：Chen L. et al.
**摘要**：通过X射线晶体学解析了重组GNAT2蛋白的三维结构，并分析了与全色盲相关的致病突变(如R84C)对蛋白构象的影响，揭示了突变导致G蛋白信号传递缺陷的分子机制。
---
3. **文献名称**：*GNAT2 knockdown in zebrafish model using recombinant antisense probes*
**作者**：Wang Y. et al.
**摘要**：利用重组GNAT2蛋白制备特异性抗体，结合反义寡核苷酸技术在斑马鱼模型中敲低GNAT2表达，证实其在视网膜发育中的关键作用，为研究色觉异常提供了体内实验依据。
---
(如需真实文献，建议在PubMed或Web of Science中检索关键词：GNAT2 recombinant protein, GNAT2 expression, achromatopsia)
**Background of GNAT2 Recombinant Protein**
GNAT2 (guanine nucleotide-binding protein G(t) subunit alpha-2) is a key component of the phototransduction cascade in cone photoreceptors, enabling color vision and daylight adaptation. As a member of the transducin family of G-protein alpha subunits, GNAT2 couples with light-activated cone opsins to initiate signaling upon photon capture. Upon activation, it exchanges GDP for GTP, dissociates from its beta-gamma counterparts, and stimulates cGMP phosphodiesterase 6 (PDE6), thereby reducing intracellular cGMP levels. This triggers the closure of cyclic nucleotide-gated (CNG) ion channels, hyperpolarizing the photoreceptor and transmitting visual signals to downstream neurons.
Mutations in the *GNAT2* gene are linked to congenital retinal disorders, such as achromatopsia (complete color blindness) and cone dystrophy, characterized by reduced visual acuity, photophobia, and loss of cone function. Studying GNAT2's structure-function relationships and signaling mechanisms is critical for understanding these pathologies and developing targeted therapies.
Recombinant GNAT2 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional properties of the native protein, including GTP-binding and PDE6 activation capabilities. Its production often involves affinity purification and refolding to ensure proper conformational integrity. Researchers employ recombinant GNAT2 to investigate molecular interactions in phototransduction, model disease-associated mutations *in vitro*, and screen potential pharmacological agents for vision disorders. Additionally, it serves as a tool for structural studies (e.g., X-ray crystallography) to elucidate mechanisms of G-protein signaling and design gene therapy vectors for retinal diseases.
Overall, GNAT2 recombinant protein bridges basic research and translational applications, offering insights into visual physiology and therapeutic innovation for inherited retinal degenerations.
×
