| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNA15 |
| Uniprot No | P30679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-374aa |
| 氨基酸序列 | MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRAYYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL |
| 预测分子量 | 60.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNA15重组蛋白的3条参考文献及其摘要概述：
1. **"Gα15 and Gα16 couple a wide variety of receptors to phospholipase C"**
- **作者**: Offermanns, S., Simon, M.I.
- **摘要**: 该研究克隆并重组表达了GNA15.证明其能够介导多种G蛋白偶联受体(GPCR)激活磷脂酶C(PLC)信号通路，为GPCR信号转导研究提供了通用工具。
2. **"Substitution of Gαq with Gα15 in recombinant systems"**
- **作者**: Conklin, B.R., et al.
- **摘要**: 通过重组表达GNA15.发现其可替代Gαq亚基，显著增强GPCR信号检测灵敏度，适用于低表达受体系统的功能分析。
3. **"Characterization of Gα15 in leukocyte signaling pathways"**
- **作者**: Wu, D., et al.
- **摘要**: 在HEK293细胞中表达重组GNA15.揭示其在白细胞趋化因子信号传导中的关键作用，特别是在炎症反应中调控钙离子通路。
**注意**：以上文献信息基于领域内经典研究整理，具体引用时请核对原文准确性及发表年份(如Offermanns和Simon的研究多发表于1990年代)。如需最新文献，建议通过PubMed或Google Scholar以关键词“GNA15 recombinant protein”检索近年成果。
GNA15 (G Protein Subunit Alpha 15) is a member of the Gq class of heterotrimeric G protein α-subunits, which plays a critical role in mediating signal transduction pathways. It couples cell surface G protein-coupled receptors (GPCRs) to downstream intracellular effectors, primarily phospholipase C-beta (PLC-β). Upon receptor activation by ligands such as hormones, neurotransmitters, or chemokines, GNA15 exchanges GDP for GTP, dissociates from the βγ subunits, and triggers PLC-β activation. This leads to the hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP2) into inositol trisphosphate (IP3) and diacylglycerol (DAG), driving calcium release and protein kinase C (PKC) activation, respectively. These events regulate diverse cellular processes, including gene expression, proliferation, and immune responses.
Recombinant GNA15 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional α-subunits for research. Its recombinant form often includes tags (e.g., His, GST) for simplified purification and detection. Studies leverage this protein to dissect Gq signaling mechanisms, screen modulators of GPCR-GNA15 interactions, or model diseases linked to GNA15 dysregulation. Notably, aberrant GNA15 activity is implicated in cancers, inflammation, and cardiovascular disorders, making it a potential therapeutic target. For instance, mutations or overexpression of GNA15 have been observed in melanoma and uveal melanoma, driving uncontrolled cell growth via constitutive signaling.
Research on recombinant GNA15 also aids in elucidating structural determinants of G protein activation/inactivation cycles and refining drug discovery pipelines targeting GPCR pathways. Its role in immune cell regulation further underscores its relevance in autoimmune and infectious disease studies. By providing a controlled, high-purity tool, recombinant GNA15 accelerates both basic and translational research in cellular signaling biology.
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