| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GK2 |
| Uniprot No | Q14410 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-553aa |
| 氨基酸序列 | AAPKTAAVGPLVGAVVQGTNSTRFLVFNSKTAELLSHHKVELTQEFPKEGWVEQDPKEILQSVYECIARTCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSKTGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNIHSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAIAGAVIRWLRDNLGIIETSGDIERLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGILCGLTQFTNKCHIAFAALEAVCFQTREILEAMNRDCGIPLRHLQVDGGMTNNKVLMQLQADILHIPVIKPFMPETTALGAAMAAGAAEGVSVWSLEPQALSVLRMERFEPQIQATESEIRYATWKKAVMKSMGWVTSQSPEGGDPSIFSSLPLGFFIVSSMVMLIGARYISGVP |
| 预测分子量 | 76.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于GK2重组蛋白的参考文献示例，供参考(实际文献需通过学术数据库获取)：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant GK2 Protein in Bacterial Systems*
**作者**：Zhang, L., et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了具有生物活性的GK2重组蛋白。通过优化表达条件，提高了蛋白溶解度，并验证其与底物结合的活性，为后续功能研究提供了基础。
2. **文献名称**：*Crystal Structure of GK2 Recombinant Protein Reveals Novel Catalytic Mechanisms*
**作者**：Wang, Y., & Tanaka, M.
**摘要**：利用X射线晶体学解析了GK2重组蛋白的三维结构，揭示了其独特的催化结构域构象，并提出了该蛋白在代谢调控中的潜在作用机制。
3. **文献名称**：*Therapeutic Potential of GK2 Recombinant Protein in Diabetes Mellitus*
**作者**：Kim, S., et al.
**摘要**：通过动物模型实验发现，外源性GK2重组蛋白可显著改善胰岛素敏感性，表明其在糖尿病治疗中的临床应用前景。
4. **文献名称**：*Development of a High-Yield Mammalian Cell System for GK2 Recombinant Protein Production*
**作者**：Garcia, R., et al.
**摘要**：构建了基于哺乳动物细胞的高效表达系统，实现了GK2重组蛋白的高产量生产，并验证了其糖基化修饰对功能的影响。
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**注意**：以上为模拟示例，实际文献需通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词(如“GK2 recombinant protein”或结合具体研究领域)获取。若GK2指代特定蛋白(如甘油激酶、激酶相关蛋白等)，建议补充全称以提高检索准确性。
GK2 recombinant protein is an engineered biomolecule derived from the glycoprotein K2 (GK2), a component of the herpes simplex virus (HSV) envelope. Initially identified for its role in viral entry and cell-to-cell spread, GK2 has garnered attention as a potential target for antiviral therapies and vaccine development. The recombinant version is typically produced using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications.
Research on GK2 focuses on its structural and functional properties. Studies reveal that GK2 interacts with host cell receptors, facilitating viral attachment and membrane fusion. Its immunogenic epitopes have been explored for eliciting neutralizing antibodies, making it a candidate for subunit vaccines against HSV infections. Additionally, GK2's role in modulating immune responses—such as evading host defenses—has spurred interest in understanding its mechanisms to design targeted interventions.
Recent advancements include optimizing GK2 recombinant protein production for enhanced stability and antigenicity. Preclinical trials demonstrate its potential to induce robust immune responses in animal models, though challenges like antigenic variability and immune evasion strategies of HSV remain hurdles. Beyond virology, GK2 recombinant protein is being repurposed in bioengineering for drug delivery systems due to its membrane fusion capabilities.
Overall, GK2 recombinant protein represents a bridge between virology and immunotherapy, offering insights into viral pathogenesis while paving the way for novel therapeutic and prophylactic tools. Ongoing research aims to refine its applications, addressing both infectious diseases and broader biomedical challenges.
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