| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FTSJ1 |
| Uniprot No | Q9UET6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MGRTSKDKRDVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQKIGGQGSGHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQLDGPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKRKGQLAKEIRPQDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMSCSP |
| 预测分子量 | 40.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FTSJ1重组蛋白的3篇代表性文献(注：文献信息为虚拟模拟，仅供参考)：
1. **文献名称**：Structural and Functional Analysis of Human FTSJ1 as a Ribosomal RNA Methyltransferase
**作者**：Ramachandran, S. et al.
**摘要**：本研究通过重组表达人源FTSJ1蛋白，解析其三维结构，并验证其作为甲基转移酶的活性。实验表明，FTSJ1重组蛋白能催化特定核糖体RNA的2'-O-甲基化修饰，突变关键催化位点会显著降低酶活性。
2. **文献名称**：FTSJ1 Deficiency Impairs tRNA Modification and Links to Intellectual Disability
**作者**：Zhang, Y. et al.
**摘要**：利用重组FTSJ1蛋白进行体外功能实验，发现其参与tRNA的甲基化修饰。通过对比患者细胞系与重组蛋白的互补实验，证明FTSJ1功能缺失导致tRNA修饰异常，可能与X染色体相关智力障碍相关。
3. **文献名称**：Recombinant FTSJ1 Expression and Its Role in Viral RNA Recognition
**作者**：Garcia-Sastre, A. et al.
**摘要**：在大肠杆菌中表达并纯化重组FTSJ1蛋白，研究其与病毒RNA的相互作用。结果表明，FTSJ1可能通过甲基化修饰宿主或病毒RNA，影响先天免疫应答通路。
4. **文献名称**：Biochemical Characterization of FTSJ1 Recombinant Protein in RNA Binding
**作者**：Chen, L. et al.
**摘要**：通过重组FTSJ1蛋白的体外结合实验，发现其特异性结合特定RNA序列，并依赖甲基转移酶结构域。研究揭示了FTSJ1在RNA代谢中的潜在分子机制。
如需具体文献，建议通过PubMed或Google Scholar以“FTSJ1 recombinant protein”“FTSJ1 methyltransferase”等关键词检索。
FTSJ1 recombinant protein is derived from the human FTSJ1 gene, which encodes a conserved S-adenosylmethionine (SAM)-dependent methyltransferase. This enzyme belongs to the seven-beta-strand methyltransferase superfamily and is implicated in post-transcriptional RNA modifications. FTSJ1 is primarily localized in the nucleus and cytoplasm, where it facilitates the 2'-O-methylation of ribosomal RNA (rRNA) and transfer RNA (tRNA), a process critical for RNA stability, translational fidelity, and cellular stress responses. Dysregulation of FTSJ1 has been linked to X-linked intellectual disability (XLID) and neurodevelopmental disorders, with pathogenic mutations disrupting its methyltransferase activity or substrate recognition.
Recombinant FTSJ1 protein is typically produced in heterologous expression systems, such as *E. coli* or mammalian cell lines, enabling large-scale purification for functional studies. The recombinant form retains key structural features, including SAM-binding domains and catalytic motifs, allowing researchers to investigate its enzymatic mechanisms and interactions with RNA substrates. Affinity tags (e.g., His-tag) are often incorporated to streamline purification via immobilized metal chromatography.
Studies leveraging recombinant FTSJ1 focus on elucidating its role in RNA modification pathways, cellular homeostasis, and disease pathogenesis. It serves as a tool for *in vitro* methylation assays, structural analyses (e.g., X-ray crystallography), and screening potential therapeutic compounds targeting RNA-modifying enzymes. Challenges in its application include optimizing solubility, preserving post-translational modifications, and mimicking native conformational states. Current research explores its involvement in antiviral responses, cancer biology, and neuronal development, highlighting its potential as a biomarker or therapeutic target for RNA-related disorders.
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