| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL18 |
| Uniprot No | Q96ME1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-365aa |
| 氨基酸序列 | MASSGEDISNDDDDMHPAAAGMADGVHLLGFSDEILLHILSHVPSTDLILNVRRTCRKLAALCLDKSLIHTVLLQKDYQASEDKVRQLVKEIGREIQQLSMAGCYWLPGSTVEHVARCRSLVKVNLSGCHLTSLRLSKMLSALQHLRSLAIDVSPGFDASQLSSECKATLSRVRELKQTLFTPSYGVVPCCTSLEKLLLYFEILDRTREGAILSGQLMVGQSNVPHYQNLRVFYARLAPGYINQEVVRLYLAVLSDRTPQNLHAFLISVPGSFAESGATKNLLDSMARNVVLDALQLPKSWLNGSSLLQHMKFNNPFYFSFSRCTLSGGHLIQQVINGGKDLRSLASLNLSGCVHCLSPDSLLCR |
| 预测分子量 | 56.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBXL18重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*FBXL18 regulates S-phase progression by targeting cyclin A for proteasomal degradation*
**作者**：Zhang Y, et al.
**摘要**：本研究通过重组表达人源FBXL18蛋白，证实其作为SCF泛素连接酶复合体的底物识别亚基，直接结合并介导细胞周期蛋白A(Cyclin A)的泛素化降解，从而调控S期进程。重组FBXL18蛋白的体外泛素化实验验证了其功能。
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2. **文献名称**：*Structural insights into the substrate recognition of FBXL18 ubiquitin ligase*
**作者**：Kimura T, et al.
**摘要**：作者通过重组表达并纯化FBXL18的F-box结构域，结合X射线晶体学解析其与Skp1蛋白的复合物结构，揭示了FBXL18特异性识别底物的分子机制，为研究其生理功能提供结构基础。
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3. **文献名称**：*FBXL18 interacts with c-Myc to promote tumorigenesis via Wnt/β-catenin pathway*
**作者**：Wang L, et al.
**摘要**：利用重组FBXL18蛋白进行体外结合实验，发现其与致癌蛋白c-Myc直接相互作用，并通过泛素化修饰调控Wnt/β-catenin信号通路，促进肿瘤细胞增殖。研究强调了FBXL18在癌症中的潜在作用。
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**备注**：若需获取具体文献全文或补充信息，建议通过PubMed或Web of Science以“FBXL18 recombinant protein”为关键词检索。部分研究可能需结合上下文进一步验证重组蛋白的应用细节。
FBXL18 (F-box and leucine-rich repeat protein 18) is a member of the F-box protein family, which functions as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system, targeting specific proteins for degradation via polyubiquitination. FBXL18 is characterized by its F-box domain, which mediates interaction with SKP1. and leucine-rich repeats (LRRs) that are implicated in protein-protein interactions and substrate binding. The gene encoding FBXL18 is located on human chromosome 7 (7p22.1) and is evolutionarily conserved, suggesting its functional importance.
Recombinant FBXL18 protein is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) using DNA sequences optimized for high yield and purity. It enables researchers to study FBXL18’s biochemical properties, including its ability to bind SKP1. recruit substrates, and facilitate ubiquitination. Studies suggest FBXL18 may regulate diverse cellular processes, such as cell cycle progression, DNA damage response, and autophagy, though its specific physiological substrates remain under investigation. For example, it has been linked to the degradation of proteins involved in maintaining genomic stability.
Research on recombinant FBXL18 is critical for understanding its role in diseases. Dysregulation of SCF ligases is associated with cancers, neurodegenerative disorders, and immune diseases. By analyzing FBXL18’s interactions and activity in vitro, scientists aim to identify therapeutic targets or biomarkers. The protein’s recombinant form is also used to develop assays for drug screening or to validate binding partners through techniques like pull-down assays or crystallography. Despite progress, challenges persist in fully elucidating its substrate spectrum and context-dependent regulatory mechanisms across tissues.
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