| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FANCC |
| Uniprot No | Q00597 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MAQDSVDLSCDYQFWMQKLSVWDQASTLETQQDTCLHVAQFQEFLRKMYEALKEMDSNTVIERFPTIGQLLAKACWNPFILAYDESQKILIWCLCCLINKEPQNSGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNMVLSLASELRENHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEALLICHGREPQEILQPEFFEAVNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSLPQAACHPAIFRVVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYFPYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHFGGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRSSSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEITHEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV |
| 预测分子量 | 79.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FANCC重组蛋白的3篇参考文献(信息基于公开论文总结，非真实文献)：
1. **文献名称**：*Functional characterization of recombinant FANCC protein in DNA crosslink repair*
**作者**：Smith J, et al.
**摘要**：本研究通过在大肠杆菌中表达并纯化重组FANCC蛋白，验证其在体外修复DNA交联损伤中的功能，发现其与FANCA/FANCG形成复合物后可增强核酸酶活性，为范可尼贫血症的分子机制提供依据。
2. **文献名称**：*Structural analysis of FANCC protein interaction with FAAP20*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析重组人源FANCC蛋白与FAAP20的复合物结构，揭示了二者结合的关键结构域，并证明该互作对范可尼贫血症相关DNA损伤修复通路的调控至关重要。
3. **文献名称**：*Recombinant FANCC delivery rescues hematopoietic stem cells in Fanconi anemia model mice*
**作者**：Wang Y, et al.
**摘要**：利用腺病毒载体递送重组FANCC蛋白至范可尼贫血症模型小鼠的造血干细胞，结果显示其可显著恢复细胞对DNA损伤因子的抵抗能力，为基因治疗提供潜在策略。
注：以上文献为示例，实际引用需根据具体研究查找真实发表论文。
**Background of Recombinant FANCC Protein**
The FANCC protein is a critical component of the Fanconi anemia (FA) pathway, a DNA repair mechanism essential for maintaining genomic stability. Mutations in the *FANCC* gene are associated with Fanconi anemia type C, a rare inherited disorder characterized by bone marrow failure, congenital abnormalities, and heightened cancer susceptibility. The FA pathway, involving a network of proteins (FANCA to FANCW), coordinates the repair of DNA interstrand crosslinks (ICLs) through homologous recombination and other DNA damage response mechanisms. FANCC, as part of the FA core complex, facilitates the monoubiquitination of FANCD2 and FANCI, a key step in recruiting downstream repair factors.
Recombinant FANCC protein is produced using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to express the purified protein for functional and structural studies. Its production enables researchers to dissect molecular interactions within the FA pathway, investigate disease-causing mutations, and explore therapeutic strategies. For example, recombinant FANCC has been used to restore DNA repair activity in FANCC-deficient cells in preclinical models, highlighting its potential in gene therapy. Additionally, it aids in studying the protein’s role in hematopoiesis and tumor suppression, as well as its interplay with other FA proteins and regulatory molecules like BRCA1/2.
Research on recombinant FANCC also contributes to understanding FA heterogeneity, as mutations in different FA genes lead to varying clinical severities. Beyond Fanconi anemia, insights from FANCC studies have implications for cancer biology, particularly in cancers with defective DNA repair mechanisms. Overall, recombinant FANCC serves as a vital tool for unraveling FA pathophysiology and advancing targeted therapies for FA and related malignancies.
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