| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ESR2 |
| Uniprot No | Q92731 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-530aa |
| 氨基酸序列 | DIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ |
| 预测分子量 | 61.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ESR2(雌激素受体β)重组蛋白的相关文献摘要概括：
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1. **文献名称**: *"Purification and characterization of recombinant human estrogen receptor β ligand-binding domain"*
**作者**: Zhang et al. (2005)
**摘要**: 研究报道了通过大肠杆菌表达系统成功表达并纯化人源ESR2配体结合域(LBD)重组蛋白，分析了其与雌激素类似物的结合活性，为结构研究和药物筛选提供基础。
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2. **文献名称**: *"Crystal structure of the human estrogen receptor β ligand-binding domain in complex with a selective agonist"*
**作者**: Pike et al. (2000)
**摘要**: 通过X射线晶体学解析了ESR2-LBD重组蛋白与选择性激动剂复合物的三维结构，揭示了ESR2与配体相互作用的分子机制，强调了其与ESR1(雌激素受体α)的结构差异。
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3. **文献名称**: *"Functional characterization of recombinant estrogen receptor β variants in hormone-responsive cell lines"*
**作者**: Leung et al. (2010)
**摘要**: 构建了多种ESR2重组蛋白突变体，通过体外细胞实验验证其转录激活功能，发现特定结构域突变显著影响受体对雌激素信号的响应能力，为ESR2功能研究提供新见解。
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注：以上文献为示例，实际引用时需核对具体来源及准确性。
**Background of ESR2 Recombinant Protein**
Estrogen receptor 2 (ESR2), also known as ERβ, is a nuclear receptor that mediates the biological effects of estrogen hormones, playing critical roles in regulating gene expression, cellular proliferation, and differentiation. It is one of two primary estrogen receptor subtypes (ESR1/ERα and ESR2/ERβ), sharing structural homology but displaying distinct tissue distribution and functional roles. ESR2 is broadly expressed in reproductive tissues, bone, brain, cardiovascular systems, and immune cells, influencing processes such as ovarian function, neuroprotection, and inflammation.
Structurally, ESR2 contains a DNA-binding domain (DBD) for target gene recognition, a ligand-binding domain (LBD) that interacts with estrogens or synthetic ligands, and activation function (AF) regions that regulate transcriptional activity. Unlike ESR1. ESR2 exhibits higher affinity for certain phytoestrogens and selective modulators, contributing to its unique regulatory pathways.
Recombinant ESR2 proteins are engineered *in vitro* using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional receptor variants. These proteins often include tags (e.g., His-tag) for affinity purification and tracking. Recombinant ESR2 enables *in vitro* studies of ligand-receptor interactions, co-regulator recruitment, and structural analyses (e.g., X-ray crystallography). It is also vital for drug discovery, particularly in developing tissue-selective estrogen therapies, and for investigating ESR2’s role in diseases such as breast cancer (where its loss correlates with poor prognosis), endometriosis, and neurodegenerative disorders.
Overall, ESR2 recombinant proteins serve as essential tools for deciphering estrogen signaling complexity and advancing targeted therapeutic strategies.
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