| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPHX2 |
| Uniprot No | P34913 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-555aa |
| 氨基酸序列 | MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM |
| 预测分子量 | 78.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPHX2(可溶性环氧化物水解酶，sEH)重组蛋白的示例参考文献，内容基于典型研究方向整理：
1. **"Cloning, expression, and purification of human soluble epoxide hydrolase (EPHX2) in Escherichia coli"**
- **作者**: Morisseau C, Hammock BD
- **摘要**: 该研究描述了在大肠杆菌中高效表达和纯化重组人sEH蛋白的方法，优化了诱导条件以提高蛋白产量。纯化后的酶通过荧光底物测定显示高水解活性，并验证了其对抑制剂的敏感性。
2. **"Crystal structure of soluble epoxide hydrolase reveals a novel dimeric interface"**
- **作者**: Argiriadi MA, et al.
- **摘要**: 通过X射线晶体学解析了重组sEH蛋白的三维结构，揭示了其独特的二聚体界面和催化位点特征，为基于结构的抑制剂设计提供了关键信息。
3. **"Role of soluble epoxide hydrolase in experimental hypertension: studies with recombinant protein and inhibitors"**
- **作者**: Imig JD, et al.
- **摘要**: 利用重组sEH蛋白探究其在高血压模型中的作用，发现抑制sEH活性可增加EETs水平，改善血管功能，提示其作为治疗靶点的潜力。
4. **"Comparative kinetic analysis of mammalian soluble epoxide hydrolases"**
- **作者**: Newman JW, et al.
- **摘要**: 比较了人、小鼠和大鼠来源的重组sEH蛋白的酶动力学差异，发现物种间底物偏好性和抑制剂敏感性存在差异，为跨物种药理学研究提供参考。
*注：以上文献为示例，实际引用时建议通过PubMed或学术数据库检索真实发表论文。*
**Background of EPHX2 Recombinant Protein**
The **epoxide hydrolase 2 (EPHX2)**, also known as soluble epoxide hydrolase (sEH), is a bifunctional enzyme encoded by the *EPHX2* gene in humans. It plays a critical role in the metabolism of endogenous and exogenous epoxides, converting bioactive epoxy fatty acids (EpFAs) into their corresponding diols. EpFAs, such as epoxyeicosatrienoic acids (EETs), are signaling molecules derived from arachidonic acid and other polyunsaturated fatty acids. These molecules regulate physiological processes, including vascular tone, inflammation, and pain response. EPHX2-mediated hydrolysis of EpFAs diminishes their biological activity, positioning the enzyme as a key modulator of lipid signaling pathways.
Structurally, EPHX2 comprises two distinct catalytic domains: an N-terminal phosphatase domain and a C-terminal epoxide hydrolase domain. The hydrolase domain is responsible for its primary enzymatic activity. Recombinant EPHX2 protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable large-scale purification for biochemical and pharmacological studies. Its recombinant form retains enzymatic activity, facilitating research into substrate specificity, inhibitor screening, and structural analysis.
EPHX2 has garnered significant attention as a therapeutic target due to its association with cardiovascular diseases, metabolic disorders, and neurodegenerative conditions. Inhibitors of EPHX2 aim to preserve beneficial EpFA levels, offering potential treatments for hypertension, diabetes, and chronic inflammation. Additionally, genetic polymorphisms in *EPHX2* have been linked to variability in drug metabolism and disease susceptibility, underscoring its clinical relevance.
Overall, recombinant EPHX2 protein serves as a vital tool for elucidating the enzyme’s role in health and disease, driving drug discovery and mechanistic studies in lipid-mediated signaling.
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