| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOK5 |
| Uniprot No | Q9P104 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MECVGTRINDISLGEPDLLATGVEREQSERFNVYLMPSPNLDVHGECALQITYEYICLWDVQNPRVKLISWPLSALRRYGRDTTWFTFEAGRMCETGEGLFIFQTRDGEAIYQKVHSAALAIAEQHERLLQSVKNSMLQMKMSERAASLSTMVPLPRSAYWQHITRQHSTGQLYRLQDVSSPLKLHRTETFPAYRSEH |
| 预测分子量 | 38.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOK5重组蛋白的示例参考文献(注：以下文献为虚构示例，实际引用时请查询真实数据库)：
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1. **标题**：*Recombinant DOK5 Protein Enhances Insulin Signaling in Skeletal Muscle Cells*
**作者**：Chen Y, et al.
**摘要**：研究通过哺乳动物细胞系统表达重组DOK5蛋白，发现其能够与胰岛素受体底物(IRS)结合，显著促进骨骼肌细胞的胰岛素信号通路活性，为糖尿病治疗提供潜在分子靶点。
2. **标题**：*Expression and Functional Analysis of DOK5 Recombinant Protein in Neuronal Development*
**作者**：Müller R, et al.
**摘要**：利用大肠杆菌表达并纯化重组DOK5蛋白，证实其通过调控TrkB受体下游的MAPK/ERK通路，促进神经元突触生长和分化，提示其在神经退行性疾病中的潜在应用。
3. **标题**：*DOK5 Recombinant Protein Binds to RET Oncogene and Modulates Cancer Proliferation*
**作者**：Sato H, et al.
**摘要**：研究发现重组DOK5蛋白可与RET酪氨酸激酶结合，抑制甲状腺癌细胞中异常激活的RET信号，为靶向抗癌药物开发提供实验依据。
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**建议**：如需真实文献，可通过PubMed、Web of Science或Google Scholar检索关键词“DOK5 recombinant protein”“DOK5 signaling”等，并筛选近年的研究论文或权威期刊文章。
**Background of DOK5 Recombinant Protein**
The DOK5 (Docking protein 5) recombinant protein is a biologically engineered molecule derived from the *DOK5* gene, which belongs to the DOK family of adaptor proteins. These proteins play critical roles in intracellular signaling by acting as scaffolds to mediate interactions between receptors, kinases, and downstream effectors. DOK5 is primarily expressed in insulin-sensitive tissues, such as skeletal muscle, adipose tissue, and the pancreas, and is notably involved in insulin signaling pathways.
Structurally, DOK5 contains a pleckstrin homology (PH) domain at its N-terminus, which facilitates membrane localization, and a phosphotyrosine-binding (PTB) domain that interacts with phosphorylated tyrosine residues on activated receptors, such as the insulin receptor (IR) or receptor tyrosine kinases (RTKs). The C-terminal region includes multiple tyrosine phosphorylation sites that recruit signaling molecules like Grb2 or SOS, linking extracellular signals to intracellular cascades, including the MAPK/ERK and PI3K/Akt pathways.
Research highlights DOK5's role in modulating insulin sensitivity and glucose homeostasis. It enhances insulin receptor signaling by stabilizing interactions between IR and downstream effectors, making it a potential target for metabolic disorders like type 2 diabetes. Conversely, aberrant DOK5 expression or mutations have been linked to impaired insulin signaling and metabolic dysfunction.
Beyond metabolism, DOK5 is implicated in neuronal development and cancer. In the nervous system, it regulates neurite outgrowth and synaptic plasticity. In oncology, DOK5 may act as a tumor suppressor or promoter depending on context, with studies showing altered expression in cancers like lung adenocarcinoma or neuroblastoma.
Recombinant DOK5 protein, produced via bacterial or mammalian expression systems, is widely used to study its structure-function relationships, interaction partners, and therapeutic potential. Its applications span *in vitro* assays, drug screening, and elucidating molecular mechanisms in metabolic and neoplastic diseases. As a research tool, it continues to provide insights into cellular signaling networks and disease pathogenesis.
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