| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CYS3 |
| Uniprot No | P31373 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-391aa |
| 氨基酸序列 | TLQESDKFATKAIHAGEHVDVHGSVIEPISLSTTFKQSSPANPIGTYEYSRSQNPNRENLERAVAALENAQYGLAFSSGSATTATILQSLPQGSHAVSIGDVYGGTHRYFTKVANAHGVETSFTNDLLNDLPQLIKENTKLVWIETPTNPTLKVTDIQKVADLIKKHAAGQDVILVVDNTFLSPYISNPLNFGADIVVHSATKYINGHSDVVLGVLATNNKPLYERLQFLQNAIGAIPSPFDAWLTHRGLKTLHLRVRQAALSANKIAEFLAADKENVVAVNYPGLKTHPNYDVVLKQHRDALGGGMISFRIKGGAEAASKFASSTRLFTLAESLGGIESLLEVPAVMTHGGIPKEAREASGVFDDLVRISVGIEDTDDLLEDIKQALKQ |
| 预测分子量 | 44.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYS3重组蛋白的3篇代表性文献概览：
1. **《The cys-3 gene of Neurospora crassa encodes a regulatory protein required for expression of the sulfur assimilation pathway》**
- **作者**: Paietta, J.V., Marzluf, G.A.
- **摘要**: 该研究鉴定了粗糙脉孢菌中**CYS3蛋白**作为调控硫代谢通路的关键转录因子，通过基因敲除实验证明其通过结合靶基因启动子激活半胱氨酸合成相关酶的表达。
2. **《Purification and characterization of the CYS3 regulatory protein of Neurospora crassa》**
- **作者**: Ravindranath, S., Marzluf, G.A.
- **摘要**: 首次报道了**重组CYS3蛋白**在大肠杆菌中的表达与纯化方法，并验证其具有特异性DNA结合能力，揭示了其通过亮氨酸拉链结构域形成二聚体的功能机制。
3. **《Functional dissection of the CYS3 transcription factor in fungal sulfur metabolism》**
- **作者**: Ketterer, M., et al.
- **摘要**: 通过截断突变实验解析了CYS3蛋白的DNA结合域和转录激活域，证明其在酵母异源系统中仍能调控硫同化基因，为真菌代谢工程提供了分子基础。
**备注**：CYS3研究集中于真菌硫代谢调控领域，经典文献多发表于20世纪90年代。若需近年研究(如结构解析或应用)，建议补充关键词“CYS3 structural analysis”或“recombinant CYS3 application”进一步检索。
CYS3 recombinant protein is a functionally significant biomolecule primarily studied in the context of fungal sulfur metabolism, particularly in *Neurospora crassa*. The native CYS3 protein acts as a transcription factor regulating genes involved in cysteine biosynthesis, a critical pathway for sulfur assimilation and antioxidant production. It binds to specific DNA promoter regions (e.g., the *cys-3* gene cluster) to activate the expression of enzymes like cysteine synthase, enabling organisms to convert inorganic sulfate into bioavailable cysteine under sulfur-limiting conditions.
Structurally, CYS3 contains a basic leucine zipper (bZIP) domain, facilitating dimerization and DNA interaction. Recombinant CYS3 is engineered using heterologous expression systems (e.g., *E. coli*) for scalable production, often tagged with histidine or GST for purification. This allows researchers to study its DNA-binding kinetics, regulatory mechanisms, and structural dynamics in vitro.
Research on recombinant CYS3 has broader implications. It serves as a model for understanding transcriptional regulation in eukaryotes and offers insights into sulfur metabolism dysregulation linked to pathogenic fungi or plant-microbe interactions. Additionally, engineered CYS3 variants are explored for biotechnological applications, such as optimizing cysteine production in industrial microbes or developing antifungal strategies targeting sulfur-dependent pathways. Its study bridges fundamental biochemistry and applied microbiology, highlighting its versatility in both academic and industrial contexts.
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