| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EEF2K |
| Uniprot No | O00418 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-725aa |
| 氨基酸序列 | MADEDLIFRL EGVDGGQSPR AGHDGDSDGD SDDEEGYFIC PITDDPSSNQ NVNSKVNKYY SNLTKSERYS SSGSPANSFH FKEAWKHAIQ KAKHMPDPWA EFHLEDIATE RATRHRYNAV TGEWLDDEVL IKMASQPFGR GAMRECFRTK KLSNFLHAQQ WKGASNYVAK RYIEPVDRDV YFEDVRLQME AKLWGEEYNR HKPPKQVDIM QMCIIELKDR PGKPLFHLEH YIEGKYIKYN SNSGFVRDDN IRLTPQAFSH FTFERSGHQL IVVDIQGVGD LYTDPQIHTE TGTDFGDGNL GVRGMALFFY SHACNRICES MGLAPFDLSP RERDAVNQNT KLLQSAKTIL RGTEEKCGSP QVRTLSGSRP PLLRPLSENS GDENMSDVTF DSLPSSPSSA TPHSQKLDHL HWPVFSDLDN MASRDHDHLD NHRESENSGD SGYPSEKRGE LDDPEPREHG HSYSNRKYES DEDSLGSSGR VCVEKWNLLN SSRLHLPRAS AVALEVQRLN ALDLEKKIGK SILGKVHLAM VRYHEGGRFC EKGEEWDQES AVFHLEHAAN LGELEAIVGL GLMYSQLPHH ILADVSLKET EENKTKGFDY LLKAAEAGDR QSMILVARAF DSGQNLSPDR CQDWLEALHW YNTALEMTDC DEGGEYDGMQ DEPRYMMLAR EAEMLFTGGY GLEKDPQRSG DLYTQAAEAA MEAMKGRLAN QYYQKAEEAW AQMEE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant expression and characterization of human eukaryotic elongation factor 2 kinase (eEF2K)"**
- **作者**: Liu X, et al.
- **摘要**: 研究描述了在大肠杆菌中重组表达人源EEF2K蛋白的过程，优化了纯化条件并验证了其激酶活性，证实重组蛋白可用于体外酶学及抑制剂筛选研究。
2. **"Structural insights into the regulation of eukaryotic elongation factor 2 kinase by pH and calcium ions"**
- **作者**: Wang Y, et al.
- **摘要**: 通过昆虫细胞表达系统获得重组EEF2K蛋白，结合晶体结构解析，揭示了钙离子和pH变化对其激酶活性的调控机制，为靶向药物设计提供结构基础。
3. **"Development of a mammalian cell-based recombinant eEF2K production system for functional studies"**
- **作者**: Chen L, et al.
- **摘要**: 构建哺乳动物细胞(HEK293)表达重组EEF2K的策略，证明该蛋白在真核系统中正确折叠并保留生理功能，适用于细胞信号通路及癌症相关研究。
4. **"Purification and biochemical characterization of eEF2K from Saccharomyces cerevisiae"**
- **作者**: Smith J, et al.
- **摘要**: 利用酵母表达系统纯化重组EEF2K，分析其ATP结合特性及底物特异性，揭示了其在真核生物翻译延伸调控中的保守性。
Eukaryotic elongation factor 2 kinase (EEF2K), also known as calmodulin-dependent protein kinase III, is a serine/threonine kinase that regulates protein synthesis by phosphorylating and inactivating eukaryotic elongation factor 2 (eEF2). This phosphorylation event slows the elongation phase of mRNA translation, particularly under stress conditions such as nutrient deprivation, hypoxia, or energy depletion. EEF2K activity is tightly linked to cellular energy status, integrating signals from pathways like AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin (mTOR), making it a critical mediator of adaptive responses to metabolic stress.
Research into EEF2K has revealed its dual role in cell survival and death. While its activity promotes energy conservation during stress by reducing protein synthesis, sustained EEF2K activation may contribute to pathological conditions, including cancer and neurodegenerative diseases. In cancer, EEF2K is often upregulated in tumors, supporting survival under hypoxia or chemotherapy by enhancing autophagy and inhibiting apoptosis. Conversely, in neurodegenerative disorders like Alzheimer’s disease, aberrant EEF2K signaling has been implicated in synaptic dysfunction and neuronal death, possibly through dysregulated calcium homeostasis or oxidative stress.
Recombinant EEF2K proteins are engineered to study its structure, kinase activity, and interactions. These tools enable researchers to dissect molecular mechanisms, screen inhibitors, and explore therapeutic targeting. For instance, inhibiting EEF2K has emerged as a potential strategy to sensitize cancer cells to treatment or mitigate neurodegeneration. However, its context-dependent roles necessitate precise modulation, highlighting the importance of recombinant systems in elucidating its complex biology. Overall, EEF2K remains a compelling subject for understanding cellular stress adaptation and developing targeted therapies.
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