| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1E |
| Uniprot No | P49674 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-416aa |
| 氨基酸序列 | MELRVGNKYRLGRKIGSGSFGDIYLGANIASGEEVAIKLECVKTKHPQLHIESKFYKMMQGGVGIPSIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGAARNPEDVDRERREHEREERMGQLRGSATRALPPGPPTGATANRLRSAAEPVASTPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVSSSDLTGRQEVSRIPASQTSVPFDHLGK |
| 预测分子量 | 49.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK1E重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "Expression and purification of recombinant human CSNK1E in Escherichia coli for structural studies"
**作者**: Zhang L, et al.
**摘要**: 报道了通过大肠杆菌表达系统高效表达人源CSNK1E重组蛋白的优化方法，采用亲和层析和凝胶过滤纯化，获得高纯度蛋白用于X射线晶体学研究。
2. **文献名称**: "Functional characterization of CSNK1E in Wnt/β-catenin signaling pathway using recombinant protein"
**作者**: Müller S, et al.
**摘要**: 利用重组CSNK1E蛋白进行体外激酶实验，证实其在Wnt信号通路中通过磷酸化DVL2调节β-catenin稳定性，揭示了其作为潜在治疗靶点的可能性。
3. **文献名称**: "Kinetic analysis of CSNK1E inhibitors using a recombinant kinase domain"
**作者**: Thompson R, et al.
**摘要**: 构建并纯化CSNK1E激酶结构域重组蛋白，通过荧光共振能量转移(FRET)技术筛选小分子抑制剂，鉴定出两种高选择性化合物。
4. **文献名称**: "Role of CSNK1E in circadian rhythm regulation: Insights from recombinant protein assays"
**作者**: Hirota T, et al.
**摘要**: 通过体外重组蛋白磷酸化实验，证明CSNK1E与PER2蛋白直接互作，并调控其稳定性，从而影响哺乳动物生物钟周期。
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**备注**：以上为模拟文献，实际文献需通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词(如 "CSNK1E recombinant protein"、"CK1epsilon expression")获取。
CSNK1E (Casein Kinase 1 Epsilon) is a serine/threonine protein kinase belonging to the highly conserved CK1 family, which plays critical roles in regulating diverse cellular processes, including signal transduction, cell cycle progression, DNA repair, and circadian rhythms. The CSNK1E gene encodes a 416-amino-acid protein characterized by a conserved kinase domain and regulatory regions that influence substrate specificity and activity. As a key modulator of Wnt/β-catenin signaling, CSNK1E phosphorylates downstream targets such as Dishevelled (DVL) and β-catenin, thereby fine-tuning developmental pathways and cellular homeostasis. It also interacts with core circadian clock components (e.g., PERIOD proteins), contributing to the precision of 24-hour biological rhythms.
Recombinant CSNK1E protein is typically produced in heterologous expression systems like *E. coli* or mammalian cells, enabling studies of its enzymatic properties, structure-function relationships, and interactions. Purification methods often involve affinity tags (e.g., GST or His-tag) to ensure high yield and purity. This engineered protein retains kinase activity and is widely used in *in vitro* assays to screen inhibitors or study phosphorylation mechanisms. Its role in diseases, particularly cancer, neurodegenerative disorders, and metabolic syndromes, has spurred interest in targeting CSNK1E therapeutically. Dysregulation of CSNK1E is linked to tumor progression via Wnt pathway activation and circadian disruption, making it a potential biomarker or drug target. Research using recombinant CSNK1E continues to unravel its regulatory networks and therapeutic relevance across physiological and pathological contexts.
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