| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREB1 |
| Uniprot No | P16220 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-341aa |
| 氨基酸序列 | MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD |
| 预测分子量 | 40.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CREB1重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *CREB1 is a critical regulator of cell cycle progression in medullary thyroid cancer cells*
**作者**: Li, Y., et al.
**摘要**: 本研究通过重组CREB1蛋白在甲状腺髓样癌细胞中的过表达实验，揭示了CREB1通过调控Cyclin D1等细胞周期相关基因的表达，促进癌细胞增殖的分子机制。实验表明重组CREB1的DNA结合活性对细胞周期进程至关重要。
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2. **文献名称**: *Recombinant CREB protein production and characterization of its phosphorylation-dependent DNA binding*
**作者**: Zhang, Q., & Montminy, M.
**摘要**: 该研究利用大肠杆菌表达系统纯化重组CREB1蛋白，并通过体外磷酸化实验证实，其DNA结合能力依赖于Ser133位点的磷酸化修饰。研究为CREB1转录活性调控提供了生化基础。
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3. **文献名称**: *Structural basis of CREB1 recognition by the transactivation domain of CBP*
**作者**: Parker, D., et al.
**摘要**: 通过X射线晶体学解析重组CREB1与转录共激活因子CBP的复合物结构，揭示了CREB1磷酸化结构域与CBP的KIX结构域相互作用的关键位点，阐明了信号依赖性基因激活的结构基础。
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**备注**：以上文献信息为示例性内容，实际文献需通过数据库(如PubMed)检索确认。如需具体文章，可补充关键词或研究场景进一步筛选。
CREB1 (cAMP response element-binding protein 1) is a ubiquitously expressed transcription factor belonging to the CREB/ATF family of leucine zipper DNA-binding proteins. It plays a central role in regulating gene expression in response to diverse cellular signals, particularly those mediated by cAMP, calcium, and growth factors. Structurally, CREB1 contains a kinase-inducible transactivation domain (KID), a DNA-binding domain, and a leucine zipper dimerization domain. Its activity is primarily regulated through phosphorylation at serine residue 133 (Ser133) by kinases such as protein kinase A (PKA), MAPK, or CaMK, which enables its interaction with coactivators like CBP/p300 to initiate transcription.
Recombinant CREB1 proteins are engineered to study its molecular functions, post-translational modifications, and interactions in vitro. These proteins are typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like GST, His, or FLAG for purification and detection. Recombinant CREB1 enables researchers to investigate DNA-binding specificity through electrophoretic mobility shift assays (EMSA), analyze phosphorylation-dependent activation mechanisms, and screen potential modulators in drug discovery.
In biomedical research, CREB1 is implicated in neuronal plasticity, memory formation, circadian rhythm regulation, and cell survival. Dysregulation of CREB1 has been associated with cancer, neurodegenerative diseases, and mood disorders, making its recombinant form valuable for developing targeted therapies. The controlled production of recombinant CREB1 ensures consistent quality for structural studies (e.g., X-ray crystallography) and high-throughput screening platforms, advancing our understanding of cAMP-responsive gene networks and their therapeutic potential.
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