| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEBPD |
| Uniprot No | P49716 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-269aa |
| 氨基酸序列 | SAALFSLDGPARGAPWPAEPAPFYEPGRAGKPGRGAEPGALGEPGAAAPAMYDDESAIDFSAYIDSMAAVPTLELCHDELFADLFNSNHKAGGAGPLELLPGGPARPLGPGPAAPRLLKREPDWGDGDAPGSLLPAQVAACAQTVVSLAAAGQPTPPTSPEPPRSSPRQTPAPGPAREKSAGKRGPDRGSPEYRQRRERNNIAVRKSRDKAKRRNQEMQQKLVELSAENEKLHQRVEQLTRDLAGLRQFFKQLPSPPFLPAAGTADCR |
| 预测分子量 | 35.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEBPD重组蛋白的参考文献示例(注：以下文献信息为假设性示例，实际文献需通过学术数据库查询)：
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1. **文献名称**: "Recombinant CEBPD Protein Production in E. coli and Its Role in Transcriptional Regulation"
**作者**: Chen Y, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达和纯化带His标签的重组CEBPD蛋白，优化了诱导条件以提高可溶性表达。纯化后的蛋白通过EMSA实验证实其特异性结合靶基因启动子的CCAAT基序，并调控炎症相关基因表达。
2. **文献名称**: "CEBPD Recombinant Protein Enhances Adipocyte Differentiation via PPARγ Activation"
**作者**: Kim S, et al.
**摘要**: 利用昆虫细胞系统表达重组CEBPD蛋白，发现其通过激活PPARγ通路促进前脂肪细胞分化为成熟脂肪细胞，揭示了CEBPD在代谢调控中的潜在作用。
3. **文献名称**: "Structural and Functional Analysis of CEBPD Using Purified Recombinant Protein"
**作者**: Wang L, et al.
**摘要**: 通过哺乳动物细胞表达系统获得高纯度CEBPD重组蛋白，结合圆二色谱和X射线晶体学分析其结构，并验证其在肿瘤细胞凋亡中的功能，为靶向药物开发提供依据。
4. **文献名称**: "CEBPD Recombinant Protein as a Tool for Studying Inflammatory Signaling"
**作者**: Müller R, et al.
**摘要**: 开发了基于HEK293细胞的CEBPD重组蛋白生产方法，证明该蛋白通过NF-κB通路调控巨噬细胞中炎症因子(如IL-6、TNF-α)的表达，为炎症性疾病机制研究提供工具。
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**建议**：实际研究中，可通过PubMed、Web of Science等平台以关键词“CEBPD recombinant protein”、“CEBPD expression and purification”检索最新文献，重点关注方法学(表达系统、纯化策略)及功能应用(如转录调控、疾病模型研究)。
CEBPD (CCAAT/Enhancer-Binding Protein Delta), a member of the C/EBP family of transcription factors, plays critical roles in regulating immune responses, cellular differentiation, and stress signaling. It binds to DNA through its conserved basic leucine zipper (bZIP) domain, modulating the expression of target genes involved in inflammation, apoptosis, and cell cycle progression. CEBPD is dynamically regulated by extracellular signals such as cytokines, hormones, and stressors, making it a key mediator in pathological conditions like cancer, metabolic disorders, and chronic inflammation.
Recombinant CEBPD protein is typically produced using expression systems (e.g., *E. coli*, mammalian cells) to enable functional studies. Its production involves cloning the CEBPD gene into expression vectors, followed by purification via affinity chromatography (e.g., His-tag or GST-tag systems). The recombinant protein retains DNA-binding activity and can be used to investigate transcriptional regulation mechanisms, protein-protein interactions, or signaling pathways in *in vitro* assays. Researchers also employ it to validate CEBPD-specific antibodies or screen small-molecule modulators for therapeutic development.
Interest in CEBPD has grown due to its dual roles in tumor suppression and promotion, depending on cellular context. It regulates genes linked to metastasis, chemoresistance, and immune evasion, highlighting its potential as a biomarker or therapeutic target. Recombinant CEBPD provides a standardized tool to dissect these complex functions, advancing studies in immunology, oncology, and metabolic disease research.
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