| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EEF1E1 |
| Uniprot No | O43324 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAAAAELSLLEKSLGLSKGNKYSAQGERQI PVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVT QVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVD LTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **EEF1E1重组蛋白** 的示例性参考文献(内容为模拟，建议通过学术数据库核实具体文献)：
---
1. **文献名称**：*Expression and Purification of Recombinant EEF1E1 Protein for Functional Studies*
**作者**：Li X, et al.
**摘要**：本研究通过大肠杆菌表达系统成功克隆并纯化了人源EEF1E1重组蛋白，优化了诱导条件以提高溶解度。通过体外酶活实验证实了其作为翻译延伸因子的GTPase激活功能，并探讨了其在细胞应激反应中的潜在作用。
2. **文献名称**：*EEF1E1 Modulates Apoptosis in Cancer Cells via Interaction with Bcl-2 Family Proteins*
**作者**：Wang Y, et al.
**摘要**：利用重组EEF1E1蛋白进行免疫共沉淀实验，发现其通过结合促凋亡蛋白Bax调控线粒体凋亡通路。研究揭示了EEF1E1在肿瘤细胞耐药性中的新机制，为靶向治疗提供理论依据。
3. **文献名称**：*Structural Characterization of EEF1E1 and Its Role in Viral RNA Translation*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了重组EEF1E1蛋白的三维结构，发现其N端结构域与病毒RNA聚合酶存在特异性互作，可能协助某些RNA病毒(如HCV)的翻译过程，提示其作为抗病毒靶点的潜力。
4. **文献名称**：*EEF1E1 Deficiency Disrupts Ribosome Biogenesis and Leads to Developmental Defects*
**作者**：Garcia-Ruiz H, et al.
**摘要**：利用CRISPR技术构建EEF1E1敲除细胞模型，结合重组蛋白回补实验，证明EEF1E1通过调控核糖体RNA加工影响胚胎发育，其缺失导致小鼠模型神经管闭合异常。
---
**注意**：以上内容为基于EEF1E1已知功能的模拟参考文献，实际文献需通过PubMed、Google Scholar等平台以关键词“EEF1E1 recombinant protein”或“EEF1E1 function”检索。如需具体文章，可提供更详细的研究方向进一步筛选。
**Background of EEF1E1 Recombinant Protein**
EEF1E1 (eukaryotic translation elongation factor 1 epsilon-1), also known as eEF1Bα, is a critical component of the eukaryotic translation elongation factor 1 complex (eEF1), which facilitates the elongation phase of protein synthesis. This protein interacts with eEF1A, catalyzing the exchange of GDP for GTP to reactivate eEF1A for subsequent rounds of aminoacyl-tRNA delivery to the ribosome. Dysregulation of EEF1E1 has been implicated in cellular stress responses, cancer progression, and neurological disorders, underscoring its importance in maintaining translational fidelity and cellular homeostasis.
Recombinant EEF1E1 protein is engineered using heterologous expression systems, such as *E. coli* or mammalian cell lines, to produce a purified, functionally active form for research. Its recombinant production enables detailed biochemical studies, including structural analysis, enzyme kinetics, and interaction mapping with ribosomal components or regulatory partners. Additionally, it serves as a tool to investigate EEF1E1's role in diseases linked to translational defects, such as certain cancers or neurodegenerative conditions.
Studies leveraging recombinant EEF1E1 have also explored its non-canonical functions, including potential involvement in DNA repair, apoptosis, and viral replication. For instance, its overexpression or knockdown in cellular models has provided insights into how translational control intersects with stress adaptation pathways.
Overall, EEF1E1 recombinant protein is a vital resource for dissecting the molecular mechanisms of protein synthesis elongation and its broader implications in health and disease.
×
