| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCAS1 |
| Uniprot No | O75363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-584aa |
| 氨基酸序列 | MGNQMSVPQRVEDQENEPEAETYQDNASALNGVPVVVSTHTVQHLEEVDLGISVKTDNVATSSPETTEISAVADANGKNLGKEAKPEAPAAKSRFFLMLSRPVPGRTGDQAADSSLGSVKLDVSSNKAPANKDPSESWTLPVAAGPGQDTDKTPGHAPAQDKVLSAARDPTLLPPETGGAGGEAPSKPKDSSFFDKFFKLDKGQEKVPGDSQQEAKRAEHQDKVDEVPGLSGQSDDVPAGKDIVDGKEKEGQELGTADCSVPGDPEGLETAKDDSQAAAIAENNNSIMSFFKTLVSPNKAETKKDPEDTGAEKSPTTSADLKSDKANFTSQETQGAGKNSKGCNPSGHTQSVTTPEPAKEGTKEKSGPTSLPLGKLFWKKSVKEDSVPTGAEENVVCESPVEIIKSKEVESALQTVDLNEGDAAPEPTEAKLKREESKPRTSLMAFLRQMSVKGDGGITHSEEINGKDSSCQTSDSTEKTITPPEPEPTGAPQKGKEGSSKDKKSAAEMNKQKSNKQEAKEPAQCTEQATVDTNSLQNGDKLQKRPEKRQQSLGGFFKGLGPKRMLDAQVQTDPVSIGPVGKSK |
| 预测分子量 | 63.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCAS1重组蛋白的3篇参考文献示例，涵盖不同研究方向：
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1. **文献名称**：*BCAS1 regulates oligodendrocyte progenitor differentiation and CNS remyelination via microtubule dynamics*
**作者**：Fard, M.K. et al.
**摘要**：通过重组BCAS1蛋白的功能实验，研究发现其通过调控微管稳定性影响少突胶质前体细胞分化和中枢神经系统髓鞘再生，为脱髓鞘疾病治疗提供新靶点。
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2. **文献名称**：*Recombinant BCAS1 promotes breast cancer cell invasion by activating PI3K-AKT signaling*
**作者**：Tanaka, S. et al.
**摘要**：利用重组BCAS1蛋白体外实验，揭示其与PI3K调控亚基p85结合并激活AKT通路，促进乳腺癌细胞侵袭转移，提示其在肿瘤进展中的关键作用。
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3. **文献名称**：*Crystal structure of human BCAS1 reveals a microtubule-binding domain essential for its function*
**作者**：Smith, J.R. et al.
**摘要**：通过重组表达BCAS1蛋白并进行X射线晶体学分析，首次解析其三维结构，发现其C端结构域直接结合微管，为理解其细胞功能提供结构基础。
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*注：上述文献为示例性内容，实际引用时需核实真实出版物信息。*
**Background of BCAS1 Recombinant Protein**
BCAS1 (Breast Carcinoma Amplified Sequence 1) is a protein-coding gene initially identified as amplified in breast cancer, suggesting its potential role in tumorigenesis. Located on chromosome 20q13.2. BCAS1 encodes a protein involved in cellular processes such as proliferation, migration, and cytoskeletal organization. Its expression is notably upregulated in certain cancers, including breast and gastric cancers, and correlates with aggressive tumor behavior and poor prognosis.
Structurally, BCAS1 contains coiled-coil domains, which mediate protein-protein interactions. Recombinant BCAS1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical and functional properties. This engineered protein retains the functional domains of native BCAS1. enabling researchers to explore its interactions with signaling pathways, such as EGFR/HER2. and its regulatory effects on cell cycle progression.
Beyond oncology, BCAS1 has recently gained attention in neuroscience. Studies reveal its transient expression in oligodendrocyte precursor cells during active myelination in the central nervous system (CNS). Recombinant BCAS1 serves as a tool to dissect its role in myelination and remyelination processes, linking it to neurodegenerative diseases like multiple sclerosis.
Therapeutic and diagnostic applications are also emerging. Recombinant BCAS1 aids in antibody production for cancer biomarker detection and in drug screening assays targeting BCAS1-associated pathways. Its dual relevance in cancer and neurobiology underscores its versatility as a research tool, bridging molecular oncology and CNS repair mechanisms.
In summary, BCAS1 recombinant protein provides a controlled platform to decode its multifaceted roles in disease, offering insights into targeted therapies and regenerative medicine strategies.
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