| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5F1A |
| Uniprot No | P25705 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-553aa |
| 氨基酸序列 | QKTGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKTRRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGSDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVVSQHQALLGTIRADGKISEQSDAKLKEIVTNFLAGFEA |
| 预测分子量 | 59.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **ATP5F1A重组蛋白** 的示例性参考文献(注：文献为假设性示例，实际研究需通过学术数据库查询)：
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1. **"Recombinant ATP5F1A expression and its role in mitochondrial energy metabolism"**
*作者：Smith J, et al.*
**摘要**：研究通过大肠杆菌系统表达并纯化重组ATP5F1A蛋白，验证其在体外ATP合成中的功能，发现其活性依赖线粒体膜电位，为酶动力学研究提供工具。
2. **"Structural insights into ATP5F1A subunit using cryo-EM analysis of recombinant protein complexes"**
*作者：Chen L, et al.*
**摘要**：利用重组ATP5F1A蛋白与ATP合酶其他亚基共表达，通过冷冻电镜解析复合物结构，揭示其在质子通道形成中的关键构象变化。
3. **"ATP5F1A mutations alter enzymatic activity: Functional validation via recombinant mutant proteins"**
*作者：Wang Y, et al.*
**摘要**：构建ATP5F1A致病突变体的重组蛋白，证明特定突变导致ATP水解活性下降，与神经退行性疾病患者线粒体功能障碍相关。
4. **"Development of an ATP5F1A-targeted drug screening platform using recombinant protein-based assays"**
*作者：Kim T, et al.*
**摘要**：基于重组ATP5F1A建立高通量药物筛选模型，筛选出小分子抑制剂，为癌症治疗中靶向能量代谢提供新策略。
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**建议**：实际研究中可通过 **PubMed** 或 **Google Scholar** 检索关键词 `ATP5F1A recombinant protein` 或 `ATP synthase subunit alpha expression`，筛选近5年文献以获取最新进展。
ATP5F1A, also known as ATP synthase F1 subunit alpha, is a critical component of the mitochondrial ATP synthase complex (Complex V), which drives the synthesis of adenosine triphosphate (ATP) during oxidative phosphorylation. This nuclear-encoded protein localizes to the mitochondrial matrix and forms the catalytic core of the F1 sector, where it facilitates the conversion of ADP to ATP using energy derived from the proton gradient across the inner mitochondrial membrane. Structurally, ATP5F1A contains a nucleotide-binding domain that interacts with ATP/ADP and a conserved G-protein-like domain essential for rotational catalysis.
The recombinant ATP5F1A protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies of ATP synthase activity, structural analysis, and disease-related investigations. Its recombinant form often includes affinity tags (e.g., His-tag) for purification and detection. Researchers utilize this protein to explore mitochondrial dysfunction linked to neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), metabolic syndromes, and cancers, where ATP5F1A expression or mutations correlate with altered energy metabolism. For instance, decreased ATP5F1A levels have been observed in age-related pathologies, while certain cancers show upregulated expression, suggesting context-dependent roles in cell survival.
Additionally, ATP5F1A serves as a tool for drug screening targeting mitochondrial bioenergetics and for developing diagnostic assays measuring ATPase activity. Its recombinant version also aids in studying pathogenic mutations associated with rare mitochondrial encephalopathies, providing insights into genotype-phenotype relationships. Recent studies highlight its potential as a biomarker for mitochondrial disorders and a therapeutic target for metabolic reprogramming in cancer. Overall, ATP5F1A recombinant protein bridges fundamental biochemistry with translational research in mitochondrial medicine.
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