| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EEF1B2 |
| Uniprot No | P24534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MGFGDLKSPA GLQVLNDYLA DKSYIEGYVP SQADVAVFEA VSSPPPADLC HALRWYNHIK SYEKEKASLP GVKKALGKYG PADVEDTTGS GATDSKDDDD IDLFGSDDEE ESEEAKRLRE ERLAQYESKK AKKPALVAKS SILLDVKPWD DETDMAKLEE CVRSIQADGL VWGSSKLVPV GYGIKKLQIQ CVVEDDKVGT DMLEEQITAF EDYVQSMDVA AFNKILEHHH HHH |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EEF1B2重组蛋白的3篇模拟参考文献示例(基于已有研究方向的推测，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Expression and Functional Analysis of Recombinant EEF1B2 in Cancer Cell Proliferation"
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组EEF1B2蛋白，发现其过表达促进肺癌细胞增殖，并通过相互作用调控PI3K/AKT信号通路，提示其在肿瘤进展中的潜在作用。
2. **文献名称**: "Structural Characterization of Human EEF1B2 and Its Isoforms by Recombinant Protein Technology"
**作者**: Zhang L, et al.
**摘要**: 利用重组表达系统获得EEF1B2两种剪接变体蛋白，通过质谱和圆二色谱分析其结构差异，揭示了不同异构体在翻译延伸复合体中的功能特异性。
3. **文献名称**: "EEF1B2 Recombinant Protein Attenuates Oxidative Stress in Neuronal Cells"
**作者**: Tanaka K, et al.
**摘要**: 在HEK293细胞中表达EEF1B2重组蛋白，发现其通过增强抗氧化酶活性减少神经元氧化损伤，为神经退行性疾病治疗提供新靶点。
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**说明**：
- 以上文献为模拟示例，实际研究需通过PubMed、Web of Science等平台检索关键词(如"EEF1B2 recombinant"或"EEF1B2 expression")。
- EEF1B2的研究多聚焦于其在癌症、翻译调控及细胞应激反应中的功能，重组蛋白常作为工具研究其生化机制。
- 建议结合具体研究方向补充检索(如特定疾病或信号通路)。
EEF1B2 (Eukaryotic Translation Elongation Factor 1 Beta 2) is a critical protein involved in the regulation of eukaryotic protein synthesis. As a subunit of the elongation factor 1 complex (eEF1), it facilitates the delivery of aminoacyl-tRNA to ribosomes during the elongation phase of translation. Specifically, EEF1B2 functions as a guanine nucleotide exchange factor (GEF), promoting the recycling of eEF1A by catalyzing the exchange of GDP for GTP, thereby maintaining the efficiency of translational elongation. This dynamic process is essential for cellular protein homeostasis and rapid adaptation to environmental or metabolic changes.
Structurally, EEF1B2 contains conserved domains critical for its GEF activity, including a central β-barrel core and surface-exposed regions mediating protein-protein interactions. Dysregulation of EEF1B2 has been implicated in various pathological conditions. Overexpression is observed in multiple cancers, such as hepatocellular carcinoma and breast cancer, where it correlates with tumor progression, metastasis, and poor prognosis. Its oncogenic potential may stem from both translation-dependent mechanisms and non-canonical roles in cell cycle regulation, apoptosis, and signaling pathways like PI3K/AKT.
Recombinant EEF1B2 proteins, typically produced in Escherichia coli or mammalian expression systems with affinity tags (e.g., His-tag), enable detailed biochemical and functional studies. These purified proteins serve as vital tools for investigating translation regulation mechanisms, screening small-molecule inhibitors, characterizing protein interactions, and developing diagnostic antibodies. Recent studies also explore its extracellular roles in immune modulation, expanding its potential therapeutic relevance beyond traditional translation-related functions.
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