| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EEF1A1 |
| Uniprot No | P68104 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK ITKSAQKAQK AK |
| 预测分子量 | 50 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EEF1A1重组蛋白的3篇模拟参考文献示例(注：文献信息为虚构，仅供格式参考)：
1. **文献名称**：Recombinant Human EEF1A1 Protein Expression and Purification in Escherichia coli
**作者**：Smith JL, et al.
**摘要**：本研究通过构建pET载体在大肠杆菌中高效表达重组人EEF1A1蛋白，优化了诱导条件和镍柱纯化步骤，获得高纯度蛋白，验证其体外翻译活性，为功能研究奠定基础。
2. **文献名称**：EEF1A1 Structural Dynamics in Cancer Cell Proliferation Revealed by Cryo-EM
**作者**：Chen R, et al.
**摘要**：利用冷冻电镜解析重组EEF1A1蛋白的构象变化，发现其GTPase结构域在肿瘤细胞异常增殖中的调控机制，为靶向EEF1A1的抗癌药物设计提供依据。
3. **文献名称**：Functional Interaction of EEF1A1 with Viral RNA Polymerases
**作者**：Wang Y, et al.
**摘要**：通过重组EEF1A1与流感病毒聚合酶的共表达实验，证明EEF1A1通过结合病毒RNA促进复制效率，揭示了宿主因子在病毒生命周期中的新作用。
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**提示**：实际研究中建议通过PubMed或Google Scholar检索关键词“recombinant EEF1A1 protein”或“EEF1A1 expression”获取真实文献。研究热点多聚焦于其在肿瘤、神经退行性疾病及病毒学中的分子机制。
EEF1A1 (eukaryotic translation elongation factor 1 alpha 1) is a ubiquitously expressed protein critical for protein synthesis in eukaryotic cells. As a core component of the elongation factor 1 complex, it facilitates the GTP-dependent delivery of aminoacyl-tRNA to the ribosome during the elongation phase of translation. Beyond its canonical role in translation, EEF1A1 exhibits diverse non-canonical functions, including cytoskeleton organization, apoptosis regulation, and viral replication modulation, making it a multifunctional protein of broad biological interest.
The recombinant EEF1A1 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper post-translational modifications. Purification methods often involve affinity chromatography tags (e.g., His-tag) followed by rigorous quality control assessments such as SDS-PAGE and mass spectrometry to confirm molecular weight (>50 kDa) and structural integrity. Recombinant versions enable standardized studies of EEF1A1's biochemical properties, interaction networks, and enzymatic activities without contamination from endogenous cellular factors.
Research applications span multiple disciplines: In cancer biology, EEF1A1 overexpression correlates with tumor progression and drug resistance. Neuroscience studies explore its role in neurodevelopmental disorders through synaptic plasticity modulation. Virologists investigate its interaction with viral proteins from pathogens like hepatitis C virus. Additionally, recombinant EEF1A1 serves as an antigen for antibody production and a tool for structural studies using cryo-EM or X-ray crystallography. Its dual functionality in translation and cellular homeostasis continues to drive interest in therapeutic targeting and mechanistic studies of protein multifunctionality.
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