| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSENEN |
| Uniprot No | Q9NZ42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-101aa |
| 氨基酸序列 | MNLERVSNEEKLNLCRKYYLGGFAFLPFLWLVNIFWFFREAFLVPAYTEQSQIKGYVWRSAVGFLFWVIVLTSWITIFQIYRPRWGALGDYLSFTIPLGTP |
| 预测分子量 | 12,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSENEN重组蛋白的3篇参考文献，涵盖其结构、功能及疾病相关性研究：
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1. **文献名称**：*Assembly of the γ-secretase complex regulates substrate processing*
**作者**：Takasugi, N., et al.
**摘要**：该研究解析了γ-分泌酶复合体中PSENEN(Pen-2)重组蛋白的结构与功能，发现Pen-2对复合体的组装及底物(如APP)的切割活性至关重要，揭示了其在阿尔茨海默病中淀粉样蛋白生成的关键作用。
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2. **文献名称**：*Pen-2 is essential for γ-secretase complex stability and trafficking*
**作者**：Luo, W., et al.
**摘要**：通过重组蛋白表达实验，证明PSENEN(Pen-2)是维持γ-分泌酶复合体稳定性及细胞膜定位的必要组分，缺乏Pen-2会导致复合体降解并抑制Aβ肽段的产生。
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3. **文献名称**：*PSENEN mutations in familial acne inversa*
**作者**：Heilig, Y., et al.
**摘要**：研究发现PSENEN基因突变与家族性反常性痤疮相关，利用重组蛋白模型揭示突变导致γ-分泌酶功能异常，进而影响Notch信号通路，为皮肤病的分子机制提供了新见解。
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4. **文献名称**：*Structural basis of γ-secretase inhibition by a PSENEN monoclonal antibody*
**作者**：Fukumori, A., et al.
**摘要**：通过冷冻电镜技术解析PSENEN重组蛋白的构象，开发特异性抗体抑制γ-分泌酶活性，为阿尔茨海默病治疗策略提供了潜在靶点。
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以上文献聚焦于PSENEN在γ-分泌酶复合体中的结构功能、疾病关联及治疗应用，涉及重组蛋白的实验模型构建与机制解析。
PSENEN (Presenilin Enhancer) is a critical component of the γ-secretase complex, a multi-subunit transmembrane protease essential for the regulated intramembrane proteolysis (RIP) of various type I membrane proteins. This complex consists of four core subunits: presenilin (PSEN, the catalytic subunit), nicastrin, PEN-2. and PSENEN. PSENEN, also known as PRESH2 or PION, stabilizes the γ-secretase complex and facilitates its maturation and enzymatic activity. It plays a pivotal role in substrate recognition and cleavage specificity, particularly in the processing of amyloid precursor protein (APP) to generate amyloid-beta (Aβ) peptides, which are implicated in Alzheimer’s disease pathogenesis.
Recombinant PSENEN protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional and structural studies. Its recombinant form enables researchers to investigate γ-secretase assembly, substrate interactions, and mutations linked to neurodegenerative disorders or cancers. For instance, PSENEN mutations have been associated with familial acne inversa and altered γ-secretase activity in Notch signaling, influencing cell differentiation and oncogenesis. By studying recombinant PSENEN, scientists aim to dissect its role in Aβ production and explore therapeutic strategies targeting γ-secretase dysregulation in Alzheimer’s disease. Additionally, it serves as a tool for drug screening to identify modulators of γ-secretase activity with potential clinical applications.
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