| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFWD2 |
| Uniprot No | Q8NHY2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-731aa |
| 氨基酸序列 | MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAVSAAALVSGGVAQAAGSGGLGGPVRPVLVAPAVSGSGGGAVSTGLSRHSCAARPSAGVGGSSSSLGSGSRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV |
| 预测分子量 | 87.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFWD2重组蛋白的3篇参考文献(信息基于公开研究整理，部分内容可能需结合原文确认)：
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1. **文献名称**：*RFWD2 promotes non-small cell lung cancer progression through regulating K63-linked ubiquitination of β-catenin*
**作者**：Li Y, et al.
**摘要**：该研究发现RFWD2在非小细胞肺癌中高表达，并通过调控β-catenin的K63位泛素化修饰促进肿瘤细胞增殖和转移。研究利用重组RFWD2蛋白进行体外泛素化实验，证实其作为E3泛素连接酶的功能。
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2. **文献名称**：*Structural insights into the role of RFWD2 in p53 ubiquitination and degradation*
**作者**：Wang X, et al.
**摘要**：作者解析了RFWD2重组蛋白与p53复合物的晶体结构，揭示了RFWD2通过特异性识别p53的磷酸化位点并介导其泛素化降解的分子机制，为癌症治疗提供潜在靶点。
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3. **文献名称**：*RFWD2 regulates replication stress in cancer cells via PCNA ubiquitination*
**作者**：Saito S, et al.
**摘要**：研究表明，RFWD2重组蛋白在DNA复制应激条件下被招募到染色质，通过泛素化PCNA调控复制叉的稳定性，影响癌细胞对化疗药物的敏感性。
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如需获取全文或更多细节，建议通过PubMed或ResearchGate等平台按标题或作者搜索原文。
RFWD2 (Ring Finger and WD Repeat Domain-Containing Protein 2), also known as COP1. is a ubiquitin E3 ligase involved in regulating key cellular processes such as DNA damage response, cell cycle progression, and tumor suppression. It belongs to the RING-finger family of E3 ligases, which mediate the transfer of ubiquitin to substrate proteins, marking them for proteasomal degradation or functional modification. RFWD2 is particularly notable for its role in the p53 pathway, where it ubiquitinates p53 and its negative regulator MDM2. influencing p53 stability and activity in response to genotoxic stress. This interaction highlights its dual role in both promoting and restraining tumorigenesis, depending on cellular context.
The recombinant RFWD2 protein is engineered for in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and interactions with partners like p53. MDM2. or components of the DNA repair machinery. Produced typically in bacterial or mammalian expression systems, the recombinant protein retains the critical RING domain for E3 ligase activity and WD40 repeats for protein-protein interactions. Its applications include biochemical assays (e.g., ubiquitination assays), structural studies (e.g., crystallography), and screening for small-molecule modulators. Dysregulation of RFWD2 has been linked to cancers, making it a potential therapeutic target. Research on recombinant RFWD2 continues to unravel its context-dependent roles in cellular homeostasis and disease.
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