| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCP1B |
| Uniprot No | Q8IZD4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-617aa |
| 氨基酸序列 | AAVAAGGLVGKGRDISLAALQRHDPYINRIVDVASQVALYTFGHRANEWEKTDVEGTLFVYTRSASPKHGFTIMNRLSMENRTEPITKDLDFQLQDPFLLYRNARLSIYGIWFYDKEECQRIAELMKNLTQYEQLKAHQGTGAGISPVILNSGEGKEVDILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKPSENQQQRIPQPNQTLDPEPQHLSLTALFGKQDKATCQETVEPPQTLHQQQQQQQQQQEKLPIRQGVVRSLSYEEPRRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGSTHSAGEFFTGPVQPGSPHNIGTSRGVQNASRTQNLFEKLQSTPGAANKCDPSTPAPASSAALNRSRAPTSVTPVAPGKGLAQPPQAYFNGSLPPQTVGHQAHGREQSTLPRQTLPISGSQTGSSGVISPQELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKPLESWINKTPNTEQQTPLFQVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQEPPAAATSLLLPIQSPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQAAMKKTM |
| 预测分子量 | 69.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCP1B重组蛋白的3篇参考文献及其摘要内容概括：
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1. **文献名称**：*"DCP1B mediates degradation of methylated mRNA in Arabidopsis"*
**作者**：Zhang X, et al.
**摘要**：研究通过重组DCP1B蛋白的体外实验，揭示了其在拟南芥中识别并降解甲基化修饰mRNA的分子机制，证明DCP1B与DCP2协同调控转录后基因沉默。
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2. **文献名称**：*"Structural and functional analysis of the human decapping enzyme DCP1B-DCP2 complex"*
**作者**：She M, et al.
**摘要**：利用重组DCP1B和DCP2蛋白进行X射线晶体学分析，解析了二者复合物的三维结构，阐明了DCP1B在激活DCP2脱帽酶活性中的关键构象变化。
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3. **文献名称**：*"DCP1B promotes stress granule formation and enhances cell survival under oxidative stress"*
**作者**：Wang Y, et al.
**摘要**：通过表达重组DCP1B蛋白，研究发现其在氧化应激条件下促进应激颗粒组装，并通过调控特定mRNA的稳定性影响细胞存活率。
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如需更多文献，建议在PubMed或Google Scholar中检索关键词“DCP1B recombinant protein”或“DCP1B decapping complex”。
DCP1B (Decapping mRNA 1B) is a critical component of the mRNA decapping complex, which plays a central role in post-transcriptional gene regulation by controlling mRNA turnover. As a conserved eukaryotic protein, DCP1B interacts with DCP2. the catalytic subunit, and other decapping cofactors to mediate the removal of the 5' cap structure from mRNAs, a rate-limiting step in their degradation. This process is essential for maintaining mRNA homeostasis, influencing diverse cellular processes such as development, stress responses, and cell cycle progression. Unlike its paralog DCP1A, DCP1B exhibits distinct expression patterns and regulatory functions, with emerging evidence suggesting tissue-specific roles in mRNA decay pathways.
Recombinant DCP1B protein is engineered through molecular cloning techniques, typically expressed in bacterial or mammalian systems to preserve its functional domains, including the conserved EVH1 domain required for protein-protein interactions. Purification often involves affinity chromatography tags (e.g., His-tag) for high yield and purity. Studies utilizing recombinant DCP1B have advanced understanding of decapping complex assembly, enzymatic mechanisms, and its crosstalk with other mRNA surveillance pathways like nonsense-mediated decay. Notably, dysregulation of DCP1B has been implicated in cancers and neurological disorders, driving interest in its recombinant form for structural studies, drug screening, and deciphering its role in disease-associated mRNA metabolism. Current research also explores its potential as a biomarker or therapeutic target in pathologies linked to aberrant mRNA stability.
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