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| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDN3 |
| Uniprot No | P14138 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-238aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSGDAGRRG VSQAPTAARS EGDCEETVAG PGEETVAGPG EGTVAPTALQ GPSPGSPGQE QAAEGAPEHH RSRRCTCFTY KDKECVYYCH LDIIWINTPE QTVPYGLSNY RGSFRGKRSA GPLPGNLQLS HRPHLRCACV GRYDKACLHF CTQTLDVSSN SRTAEKTDKE EEGKVEVKDQ QSKQALDLHH PKLMPGSGLA LAPSTCPRCL FQEGAP |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EDN3(Endothelin-3)重组蛋白的3篇参考文献示例，基于公开研究内容概括：
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1. **文献名称**：*Recombinant EDN3 promotes differentiation of human pluripotent stem cells into enteric neural crest-like cells*
**作者**：Lui, K.N. et al.
**摘要**：研究通过在大肠杆菌中表达重组EDN3蛋白，验证其对人多能干细胞向肠神经嵴样细胞分化的促进作用。实验表明，EDN3与Wnt信号通路协同调控SOX10等标记基因表达，为肠神经相关疾病模型构建提供工具。
2. **文献名称**：*Expression and functional characterization of endothelin-3 in melanocyte development*
**作者**：Garcia, R.J. & Leachman, S.A.
**摘要**：利用哺乳动物细胞系(CHO)表达重组EDN3蛋白，证明其通过结合内皮素受体(EDNRB)激活MAPK通路，促进黑色素前体细胞迁移与存活，为色素障碍疾病机制研究提供依据。
3. **文献名称**：*Optimization of EDN3 recombinant protein production in Pichia pastoris for Hirschsprung's disease therapy*
**作者**：Chen, X. et al.
**摘要**：通过毕赤酵母系统高效表达具有生物活性的重组EDN3蛋白，优化发酵条件使产量提高3倍。动物实验显示该蛋白可改善赫什朋病模型小鼠的肠道神经节发育，提示其治疗潜力。
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**说明**：以上文献为示例性概括，实际文献需通过PubMed、Google Scholar等平台检索关键词“Recombinant EDN3 protein”或“Endothelin-3 expression”获取。
Endothelin-3 (EDN3) is a 21-amino acid peptide belonging to the endothelin family, which includes EDN1 and EDN2. It is encoded by the *EDN3* gene and functions as a potent vasoconstrictor, though its roles extend beyond cardiovascular regulation. Structurally, EDN3 shares a conserved C-terminal motif with other endothelins but differs in its N-terminal residues, conferring receptor-binding specificity. It primarily activates the endothelin receptor type B (EDNRB), a G protein-coupled receptor critical for developmental processes, including neural crest cell migration and differentiation.
Recombinant EDN3 is produced via genetic engineering in systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research and therapeutic applications. Its production involves cloning the *EDN3* gene into expression vectors, followed by purification using affinity chromatography. This recombinant form retains native functionality, enabling studies on EDN3’s signaling pathways and interactions.
Biologically, EDN3 is essential in embryogenesis, particularly in the development of enteric neurons, melanocytes, and the cardiac outflow tract. It promotes cell proliferation, survival, and differentiation through EDNRB-mediated activation of MAPK and PI3K pathways. Deficiencies in EDN3 or EDNRB are linked to Hirschsprung disease (aganglionosis of the colon) and Waardenburg syndrome (pigmentation and hearing defects), highlighting its role in neural crest biology.
In research, recombinant EDN3 is used to model these disorders, screen therapeutics, and explore tissue regeneration. Clinically, it holds potential for treating neural crest-related pathologies, though challenges in delivery and receptor specificity remain. Overall, EDN3’s multifaceted roles in development and disease make it a critical focus for both basic science and translational medicine.
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