| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | yopE |
| Uniprot No | P31492 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MKISSFISTSLPLPASVSGSSSVGEMSGRSVSQQKSDQYANNLAGRTESPQGSSLASRIIERLSSMAHSVIGFIQRMFSEGSHKPVVTPALTPAQMPSPTSFSDSIKQLAAETLPKYMQQLSSLDAETLQKNHDQFATGSGPLRGSITQCQGLMQFCGGELQAEASAILNTPVCGIPFSQWGTVGGAASAYVASGVDLTQAANEIKGLGQQMQQLLSLM |
| 预测分子量 | 26.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YopE重组蛋白的3篇代表性文献摘要概述：
1. **"YopE of Yersinia, a GAP for Rho GTPases, selectively modulates Rac-dependent actin structures in endothelial cells"**
*作者：Andor, K. et al.*
摘要：该研究解析了耶尔森菌YopE蛋白作为Rho GTP酶激活蛋白(GAP)的功能，发现其通过特异性抑制宿主细胞Rac信号通路，破坏内皮细胞骨架结构，促进细菌逃逸免疫应答。
2. **"Recombinant YopE protein as a subunit vaccine candidate against plague"**
*作者：Zhang, Y. et al.*
摘要：评估重组YopE蛋白联合其他抗原的疫苗效果，证明其在小鼠模型中诱导Th1型免疫应答，显著提高对鼠疫杆菌攻毒的保护率，提示其作为亚单位疫苗的潜力。
3. **"Structural basis of YopE recognition by the zinc-binding domain of the Yersinia outer protein kinase A (YpkA)"**
*作者：Prehna, G. et al.*
摘要：通过X射线晶体学解析YopE与YpkA锌结合结构域的复合物结构，揭示Yersinia效应蛋白间的相互作用机制，为靶向细菌分泌系统的药物设计提供依据。
注：以上文献信息为示例性质，实际研究中建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Web of Science核对最新研究进展。
The YopE protein is a key virulence factor produced by pathogenic *Yersinia* species, including *Y. pestis* (plague), *Y. pseudotuberculosis*, and *Y. enterocolitica*. It is a type III secretion system (T3SS) effector protein critical for bacterial pathogenesis. During infection, *Yersinia* delivers YopE directly into host cells to disrupt immune defenses. YopE functions as a GTPase-activating protein (GAP) targeting Rho-family GTPases (e.g., RhoA, Rac1. Cdc42), which regulate cytoskeletal dynamics. By inactivating these GTPases, YopE induces actin cytoskeleton collapse, inhibiting phagocytosis by immune cells and facilitating bacterial survival in host tissues.
Recombinant YopE is produced through genetic engineering, typically by cloning the *yopE* gene into expression vectors (e.g., *E. coli* systems) for large-scale purification. Researchers use recombinant YopE to study its structure-function relationships, host-pathogen interactions, and immune evasion mechanisms. Its role in suppressing inflammatory signaling pathways, such as MAPK and NF-κB, has also been explored. Additionally, recombinant YopE serves as a tool to investigate bacterial effector delivery mechanisms via T3SS and as a potential target for antivirulence therapies. Studies often employ mutant variants (e.g., GAP-inactive forms) to dissect enzymatic versus structural roles in infection models. Due to its immunogenicity, YopE has been investigated in vaccine development against *Yersinia* infections. Overall, recombinant YopE remains integral to understanding bacterial pathogenesis and developing countermeasures against *Yersinia*-associated diseases.
×
