| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UL49 |
| Uniprot No | P89468 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 170-300aa |
| 氨基酸序列 | QGLAKKLHFSTAPPSPTAPWTPRVAGFNKRVFCAAVGRLAATHARLAAVQLWDMSRPHTDEDLNELLDLTTIRVTVCEGKNLLQRANELVNPDAAQDVDATAAARGRPAGRAAATARAPARSASRPRRPLE |
| 预测分子量 | 17.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UL49重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**: "Structural and functional analysis of the pseudorabies virus UL49 protein"
**作者**: Zhang et al.
**摘要**: 本研究通过重组表达伪狂犬病毒(PRV)UL49蛋白，分析了其三维结构及与病毒DNA的相互作用，揭示了UL49在病毒衣壳组装中的关键作用，并发现其C端结构域对病毒粒子稳定性至关重要。
2. **文献名称**: "Characterization of the herpes simplex virus type 1 UL49 gene product as a virion tegument component"
**作者**: Kato et al.
**摘要**: 通过重组UL49蛋白的体外表达及抗体标记实验，证实HSV-1 UL49蛋白为病毒皮层(tegument)的重要组成成分，参与病毒粒子释放过程，并可能与宿主细胞膜蛋白存在相互作用。
3. **文献名称**: "Recombinant UL49 protein of human cytomegalovirus induces immune responses in a mouse model"
**作者**: Liu et al.
**摘要**: 利用重组人巨细胞病毒(HCMV)UL49蛋白进行小鼠免疫实验，发现其能诱导强烈的体液和细胞免疫反应，提示UL49或可作为候选亚单位疫苗靶点，用于抗病毒免疫策略开发。
(注：以上文献信息为示例，实际研究中需根据具体病毒及实验方向检索相关文献。)
**Background of UL49 Recombinant Protein**
UL49 is a gene product of herpesviruses, notably herpes simplex virus (HSV), belonging to the *Herpesviridae* family. It encodes a tegument protein, a structural component located between the viral capsid and envelope, crucial for viral assembly, host interactions, and immune evasion. The UL49 protein, often referred to as VP22 in HSV-1. plays roles in viral morphogenesis, intracellular trafficking, and modulation of host cell environments. Its ability to bind viral DNA and interact with other tegument proteins, such as VP16 (UL48), highlights its importance in viral replication and pathogenesis.
Recombinant UL49 protein is generated through genetic engineering, typically by cloning the UL49 gene into expression vectors (e.g., bacterial, insect, or mammalian systems) to produce purified protein for research. Studies leveraging recombinant UL49 focus on elucidating its structural and functional properties. For instance, structural analyses via X-ray crystallography or cryo-EM have revealed its DNA-binding domains and interaction interfaces, while functional assays explore its role in viral egress and immune modulation.
Research on UL49 also extends to biomedical applications. As a recombinant antigen, it serves in diagnostic kits to detect herpesvirus-specific antibodies. Additionally, it is investigated as a vaccine candidate to elicit protective immune responses. Beyond virology, UL49’s cell-penetrating properties have sparked interest in drug delivery systems. However, challenges remain in understanding its precise mechanisms in immune evasion and its variability across herpesvirus species.
Overall, UL49 recombinant protein is a vital tool for dissecting herpesvirus biology, advancing diagnostics, and developing antiviral strategies, underscoring its significance in both basic and applied virology research.
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