| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | ORF1a |
| Uniprot No | Q00020 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-966aa |
| 氨基酸序列 | MSSFVNLESLISERGANCRGADEIVNNETTRILTSQIEHSQRSKKVNIRNKLSVAECDAFRARYGGAFDVNLTHEYTAPHSLAGALRVAEHYDCIDSFPPEDKIIDFGGSWLHHYSRGDSRVHSCCPILGPRDATRHEERMCRLRKMVQTSDRFVDVPDFCLNKAEDCNVQADWAICIHGGYDMGFQGLCKAMHAPLERGILQGTIMFDGAMLFDRQGELPLLQCRWQRVGTGSKEQIKFDFINESTLSYVHDWKNLGSFLTESTYSIGGTTYLLERMLLKCSIMTYKIIATNVRCPPESLRHCIWFENISQYLAVQIPIGYNLNDWKTVRVARATVREVEEISFRCFKENKDWTENMRSVASILSAKSSTVIINGQSIMSGERLDVLEYHLVAFSLTLNLYQKYEKLRNFQGELEWKGWANHFKTRLWWCGRTVSTEGGFLRNFLADKIPWLKLNTYADSLDFITKISEVESFEVDSVPTSRLRSFFQKEENIVERAASEIMSANARRIAKKAEMSKEFDDFVDAPEEFAPEDVVEEVINTPVTQDVKLRQSKPETARSIVLDPDAVLKNGAINEFADYSKRLHENTVSNLRHLWTLMGCRGNEIHNKSVAETYHRVDDMVNVHFPNGHWMYPLKYEYTVGYNDGGLGEKFENELYVVDKTCSCANAKAIADACKKVSAPTCSVVMVDGVAGCGKTTAIKETFRFEKDIIVTANRKSAEDVRKAIFGDASDSEVALKVVRTADSAIMHGLPECHRLLVDEAGLLHYGQLLAVADLCKCSEVLAFGDTEQISFKSRDATFRMKYCNIEYDKRDIVSKTFRCPQDVVSAVKILKRKCANRSSKYNGWVSSSKVEKSLSKSRIVSINQVSMEKHKFYLTMTEADKAALCSRAKDVGLDKTWVESNMETVHEAQGKAVDHVVLVRLKSTKCDLFKSEEYCLVALTRHKRTFEYLYNGDLGGDLISFYVT |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ORF1a重组蛋白的3篇代表性文献示例(注：文献为假设性示例，实际引用需根据具体研究检索)：
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1. **文献名称**：*Structural insights into SARS-CoV-2 ORF1a polyprotein processing and replication complex assembly*
**作者**：Yang, X. et al.
**摘要内容**：该研究利用冷冻电镜技术解析了SARS-CoV-2 ORF1a重组蛋白的分子结构，揭示了其通过自切割产生非结构蛋白(如nsp3-nsp8)的机制，并阐明了其在病毒复制酶复合体组装中的关键作用。
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2. **文献名称**：*Functional characterization of ORF1a-encoded proteases in coronavirus replication*
**作者**：Zhang, L. et al.
**摘要内容**：通过体外重组表达ORF1a蛋白的蛋白酶结构域(PLpro和3CLpro)，作者验证了其切割病毒多蛋白的功能，并证明抑制剂靶向这些蛋白酶可有效阻断病毒复制，为抗冠状病毒药物开发提供了依据。
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3. **文献名称**：*Comparative analysis of ORF1a recombination events in alphacoronaviruses and betacoronaviruses*
**作者**：Smith, R. et al.
**摘要内容**：研究通过基因重组实验和系统发育分析，揭示了不同冠状病毒属(如α和β属)中ORF1a基因的重组热点区域，表明其重组可能影响宿主适应性和病毒致病性。
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如需真实文献，建议通过PubMed或Google Scholar以关键词“ORF1a recombinant protein”、“coronavirus ORF1a processing”等检索近年研究。
The ORF1a recombinant protein is a key component derived from the open reading frame 1a (ORF1a) of coronaviruses, including SARS-CoV-2. ORF1a, along with ORF1b, forms the viral replicase gene, which is translated into polyproteins pp1a and pp1ab (via a -1 ribosomal frameshift). These polyproteins undergo proteolytic processing by viral proteases to generate nonstructural proteins (nsps) essential for viral replication and transcription. ORF1a specifically encodes nsps 1-11. which include critical enzymes like the papain-like protease (PLpro, nsp3) and the main protease (Mpro, nsp5), as well as domains involved in membrane remodeling and immune evasion.
Recombinant ORF1a proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce individual nsps or functional domains for research and therapeutic applications. They are pivotal in studying viral mechanisms, such as RNA synthesis, protein processing, and host-pathogen interactions. For example, PLpro and Mpro are targets for antiviral drug development due to their roles in polyprotein cleavage and immune modulation. Recombinant versions enable high-throughput screening of inhibitors and structural studies (e.g., X-ray crystallography, cryo-EM) to guide drug design.
Additionally, ORF1a proteins aid in serological assays, detecting antibodies in infected individuals, and in elucidating viral evolution. Mutations in ORF1a (e.g., nsp6 or nsp3 regions) may influence viral fitness or clinical outcomes, making recombinant proteins valuable for functional characterization of variants. Challenges include maintaining proper folding and post-translational modifications in heterologous systems, which can affect protein activity. Overall, ORF1a recombinant proteins serve as indispensable tools for advancing coronavirus research, diagnostics, and therapeutic strategies.
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