| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UBTF |
| Uniprot No | P17480 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-670aa |
| 氨基酸序列 | MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTT |
| 预测分子量 | 80.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UBTF(上游结合转录因子)重组蛋白的3篇参考文献示例，涵盖其功能、结构及应用研究：
1. **文献名称**：*"Recombinant UBTF Purification and its Role in rRNA Transcription Activation"*
**作者**：Smith J., Doe R., et al.
**摘要**：本研究报道了人源UBTF重组蛋白在大肠杆菌中的高效表达及纯化策略，并利用体外转录实验证实UBTF通过促进RNA聚合酶I与rDNA启动子的结合，显著增强核糖体RNA的转录活性。
2. **文献名称**：*"Structural Insights into UBTF-DNA Interaction via Cryo-EM Analysis"*
**作者**：Chen L., Wang H., et al.
**摘要**：通过冷冻电镜技术解析了重组UBTF蛋白与rDNA启动子复合物的高分辨率结构，揭示了UBTF的HMG结构域如何通过DNA弯曲和染色质重塑激活转录起始复合物的组装。
3. **文献名称**：*"UBTF Recombinant Variants in Cancer: Dysregulation of Ribosomal Biogenesis"*
**作者**：Garcia M., Lee K., et al.
**摘要**：探讨了UBTF基因在多种癌症中的异常扩增现象，利用重组UBTF突变体进行功能研究，发现其过表达导致核仁结构异常和核糖体生物合成失控，为肿瘤靶向治疗提供潜在靶点。
*注：上述文献为示例性质，实际引用时需核实具体论文信息。建议通过PubMed或Web of Science以“UBTF recombinant protein”、“UBF expression”等关键词检索最新研究。*
UBTF (Upstream Binding Transcription Factor), also known as UBF, is a conserved DNA-binding protein critical for ribosomal RNA (rRNA) synthesis in eukaryotes. It functions as a key regulatory component of the RNA polymerase I (Pol I) transcription machinery, which drives the transcription of rRNA genes located in the nucleolar organizer regions (NORs). UBTF contains multiple HMG (high-mobility group) box domains that enable sequence-independent DNA bending and binding, facilitating the assembly of the transcription pre-initiation complex on ribosomal DNA (rDNA) promoters. By interacting with the SL1/TIF-IB complex and other factors, UBTF stabilizes Pol I recruitment and enhances transcriptional activation.
Recombinant UBTF proteins are widely engineered for functional and structural studies. These proteins are typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., insect or mammalian cells) to preserve post-translational modifications, such as phosphorylation, which regulates its activity. Tagged versions (e.g., His, FLAG, or GST tags) are purified for *in vitro* assays, including chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSA), and cryo-EM structural analyses. Research using recombinant UBTF has elucidated its role in nucleolar organization, rDNA chromatin remodeling, and cell cycle-dependent regulation of ribosome biogenesis.
Dysregulation of UBTF is linked to pathologies like cancer, where hyperactive rRNA synthesis supports uncontrolled cell proliferation. Recombinant UBTF also aids in studying ribosomopathies and developmental disorders. Its modular domain structure and functional versatility make it a focal point in understanding the interface between transcription regulation, epigenetics, and disease mechanisms.
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