| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Sting1 |
| Uniprot No | Q86WV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 135-379aa |
| 氨基酸序列 | GLKGLAPAEISAVCEKGNFNVAHGLAWSYYIGYLRLILPELQARIRTYNQHYNNLLRGAVSQRLYILLPLDCGVPDNLSMADPNIRFLDKLPQQTGDHAGIKDRVYSNSIYELLENGQRAGTCVLEYATPLQTLFAMSQYSQAGFSREDRLEQAKLFCRTLEDILADAPESQNNCRLIAYQEPADDSSFSLSQEVLRHLRQEEKEEVTVGSLKTSAVPSTSTMSQEPELLISGMEKPLPLRTDFS |
| 预测分子量 | 29.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STING1重组蛋白的3-4篇模拟参考文献示例(基于典型研究主题，非真实文献)：
1. **文献名称**：*"Recombinant STING1 Protein Production and Its Role in Innate Immune Signaling"*
**作者**：Chen L, et al.
**摘要**：研究利用昆虫细胞表达系统成功制备了高纯度STING1重组蛋白，并证明其在体外能有效激活干扰素β(IFN-β)通路，揭示了STING1与TBK1激酶的相互作用机制。
2. **文献名称**：*"Crystallization and Functional Analysis of Human STING1 Recombinant Protein"*
**作者**：Shimizu T, et al.
**摘要**：通过大肠杆菌表达并纯化STING1胞外结构域重组蛋白，解析其与环二核苷酸(CDNs)结合的晶体结构，阐明了配体诱导STING1二聚化的分子基础。
3. **文献名称**：*"STING1 Recombinant Protein Enhances Antiviral Response in Macrophages"*
**作者**：Wang Y, et al.
**摘要**：研究发现外源性STING1重组蛋白可激活巨噬细胞中IRF3磷酸化，显著抑制RNA病毒复制，为抗病毒治疗提供了潜在策略。
4. **文献名称**：*"Optimization of STING1 Recombinant Protein Stability for Drug Screening"*
**作者**：Garcia-Ruiz E, et al.
**摘要**：开发了一种哺乳动物细胞表达系统生产的STING1重组蛋白，并优化其稳定性，成功应用于高通量筛选靶向STING通路的激动剂/抑制剂。
**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词“STING1 recombinant protein”或“STING recombinant protein expression”获取。如需具体文献，建议结合研究领域(如结构、免疫应用或药物开发)进一步筛选。
STING1 (Stimulator of Interferon Genes), also known as TMEM173. is a central adaptor protein in the innate immune system that plays a critical role in detecting cytosolic DNA and initiating downstream signaling pathways. It is activated by cyclic dinucleotides (CDNs), such as cyclic GMP-AMP (cGAMP), produced by the enzyme cGAS in response to cytosolic DNA derived from pathogens or damaged cellular components. Upon activation, STING1 translocates from the endoplasmic reticulum to the Golgi apparatus, recruiting kinases like TBK1 and IKK, which phosphorylate transcription factors such as IRF3 and NF-κB. This cascade ultimately triggers the production of type I interferons (IFNs) and pro-inflammatory cytokines, essential for antiviral and antitumor immunity.
Recombinant STING1 protein is engineered in vitro using expression systems like bacterial or mammalian cells to study its structure, function, and interactions. It typically retains key domains, including the transmembrane regions and the C-terminal tail responsible for ligand binding and signaling. Researchers utilize recombinant STING1 to investigate mechanisms of immune activation, screen for agonists/antagonists, or develop therapeutics targeting autoimmune disorders, infections, or cancers. Dysregulated STING1 signaling is linked to diseases like systemic lupus erythematosus (SLE) and Aicardi-Goutières syndrome, making it a focus for drug discovery. Recent studies also explore its role in cancer immunotherapy, where STING1 activation enhances antitumor T-cell responses. The development of recombinant STING1 has significantly advanced both basic research and clinical applications in immunology.
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