Recombinant Human HTR2B protein

中文名： 5-羟色胺受体2B(HTR2B)重组蛋白
别名： HTR2B；5-hydroxytryptamine receptor 2B

货号: PA2000-1816

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	HTR2B
Uniprot No	P41595
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-481aa
氨基酸序列	MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQ GNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVAD LLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIMHLCAIS VDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVD NPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYL VKNKPPQRLTWLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDET LMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLC DSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYR ATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRS STIQSSSIILLDTLLLTENEGDKTEEQVSYV
预测分子量	70 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下为3篇关于HTR2B重组蛋白的参考文献及摘要概要：

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1. **文献名称**：*Structural basis for molecular recognition of serotonin by GPCRs*

**作者**：Wacker D. et al.

**摘要**：该研究通过X射线晶体学解析了人源HTR2B重组蛋白与抗偏头痛药物的复合物结构，揭示了5-HT受体特异性配体结合的分子机制，为靶向药物设计提供结构基础。

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2. **文献名称**：*Functional characterization of recombinant human 5-HT2B receptor signaling in vitro*

**作者**：Nebigil C.G. et al.

**摘要**：通过哺乳动物细胞系统表达HTR2B重组蛋白，证实其激活后通过Gq蛋白通路诱导细胞内钙离子释放，并发现该受体在心脏肥大病理模型中的异常信号传导作用。

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3. **文献名称**：*High-yield purification and biophysical analysis of human 5-HT2B receptor for drug discovery*

**作者**：Wang C. et al.

**摘要**：优化了HTR2B重组蛋白在昆虫细胞中的表达与纯化流程，获得高纯度蛋白用于表面等离子体共振(SPR)分析，筛选出新型小分子抑制剂并验证其结合活性。

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每篇文献均聚焦于HTR2B重组蛋白的不同研究维度(结构、功能、应用)，可为相关研究提供技术及理论参考。

背景信息

The 5-hydroxytryptamine receptor 2B (HTR2B) is a G protein-coupled receptor (GPCR) that binds serotonin (5-HT), a neurotransmitter and hormone involved in regulating mood, cognition, and peripheral physiological processes. HTR2B is primarily expressed in the cardiovascular system, gastrointestinal tract, and central nervous system. It mediates cellular responses through Gq/11 protein signaling, leading to phospholipase C activation, intracellular calcium release, and downstream pathways influencing vascular contraction, cardiac function, and neural activity. Dysregulation of HTR2B has been implicated in conditions such as pulmonary hypertension, heart valve disease, and neuropsychiatric disorders.

Recombinant HTR2B protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional receptor variants for research and drug development. Its recombinant form often includes tags (e.g., His-tag) for simplified purification and detection. Structural studies of HTR2B recombinant proteins have advanced understanding of ligand-binding mechanisms, receptor activation, and interactions with antagonists like ergot derivatives or selective serotonin reuptake inhibitors (SSRIs). This protein is critical for high-throughput screening of potential therapeutics targeting serotonin-related pathologies. Additionally, it aids in elucidating HTR2B's role in developmental biology, as studies suggest its involvement in embryonic heart formation. However, challenges persist in maintaining receptor stability and native conformation during recombinant production, necessitating optimized solubilization and membrane mimicry techniques. Current research leverages cryo-EM and X-ray crystallography to resolve HTR2B's 3D structure, facilitating precision drug design while minimizing off-target effects linked to serotonin receptor subtypes.