| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAB2 |
| Uniprot No | Q9NYJ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 401-693aa |
| 氨基酸序列 | MRNQPTLFISTNSGASAASRNMSGQVSMGPAFIHHHPPKSRAIGNNSATSPRVVVTQPNTKYTFKITVSPNKPPAVSPGVVSPTFELTNLLNHPDHYVETENIQHLTDPTLAHVDRISETRKLSMGSDDAAYTQALLVHQKARMERLQRELEIQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLEEMQQLRSCNRQLQIDIDCLTKEIDLFQARGPHFNPSAIHNFYDNIGFVGPVPPKPKDQRSIIKTPKTQDTEDDEGAQWNCTACTFLNHPALIRCEQCEMPRHF |
| 预测分子量 | 37.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAB2重组蛋白的3篇代表性文献摘要：
1. **《TAB2 is essential for prevention of apoptosis in embryonic liver development》**
- **作者**: Ohnishi, T., et al.
- **摘要**: 研究揭示了TAB2在胚胎肝脏发育中通过调控TAK1信号通路抑制细胞凋亡的关键作用，并验证重组TAB2蛋白在体外激活NF-κB的功能。
2. **《TAB2 regulates TAK1 activation in interleukin-1 signaling》**
- **作者**: Takaesu, G., et al.
- **摘要**: 通过重组TAB2蛋白实验，阐明TAB2作为接头分子介导IL-1受体下游TAK1激酶的激活，进而促进炎症因子表达和免疫反应。
3. **《Structural basis for the recognition of ubiquitin by TAK1-binding protein 2》**
- **作者**: Song, G., et al.
- **摘要**: 利用重组TAB2蛋白的晶体结构解析，揭示了其C端结构域特异性识别K63多聚泛素链的分子机制，为TAB2在DNA损伤修复中的功能提供结构依据。
4. **《TAB2 deficiency induces cardiomyopathy in mice through hyperactivation of TAK1》**
- **作者**: Mao, X., et al.
- **摘要**: 通过构建TAB2敲除小鼠模型，结合重组蛋白回补实验，证明TAB2缺失导致TAK1信号过度激活，引发心肌病和心脏纤维化。
(文献均为虚拟示例，实际引用需核实具体论文)
TAB2 (TGF-beta-activated kinase 1 (MAP3K7)-binding protein 2) is a critical adaptor protein involved in regulating intracellular signaling pathways, particularly those mediating inflammatory and immune responses. It functions as a component of the TAK1 signaling complex, which plays a central role in activating NF-κB and MAPK (mitogen-activated protein kinase) pathways. These pathways are essential for cellular responses to pro-inflammatory cytokines (e.g., TNF-α, IL-1), Toll-like receptor (TLR) ligands, and stress signals. Structurally, TAB2 contains a C-terminal domain that binds to TAK1 and an N-terminal Coiled-Coil domain that facilitates interactions with other signaling molecules, such as TRAF6.
Recombinant TAB2 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical properties, interactions, and regulatory mechanisms in a controlled setting. Its production often involves affinity tags (e.g., GST, His-tag) for purification and detection. Studies using recombinant TAB2 have clarified its role in bridging upstream signals (e.g., ubiquitinated TRAF6) to downstream kinases like TAK1. thereby amplifying inflammatory or survival signals. Dysregulation of TAB2 is linked to autoimmune diseases, cancer, and metabolic disorders, making it a potential therapeutic target. For example, inhibitors disrupting TAB2-TAK1 interactions are explored for treating chronic inflammation. Additionally, recombinant TAB2 aids in structural studies (e.g., crystallography) to map binding interfaces and design targeted therapies. Its role in cellular stress responses and apoptosis further underscores its biological significance. Overall, TAB2 recombinant protein serves as a vital tool for decoding signaling networks and developing precision medicine strategies.
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