| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C1ql3 |
| Uniprot No | Q5VWW1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-255aa |
| 氨基酸序列 | HYEMLGTCRM VCDPYGGTKA PSTAATPDRG LMQSLPTFIQ GPKGEAGRPG KAGPRGPPGE PGPPGPMGPP GEKGEPGRQG LPGPPGAPGL NAAGAISAAT YSTVPKIAFY AGLKRQHEGY EVLKFDDVVT NLGNHYDPTT GKFTCSIPGI YFFTYHVLMR GGDGTSMWAD LCKNNQVRAS AIAQDADQNY DYASNSVVLH LEPGDEVYIK LDGGKAHGGN NNKYSTFSGF IIYAD |
| 预测分子量 | 26,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C1ql3重组蛋白的3篇参考文献及摘要概括：
1. **"C1ql3 regulates cortical circuit development by mediating synapse formation"**
*作者：Martin et al. (2020)*
摘要：研究通过重组C1ql3蛋白验证其在皮质神经元突触形成中的作用，发现重组C1ql3通过与Bai3受体结合促进兴奋性突触发育，并解析了其重组蛋白的体外功能活性。
2. **"Structural insights into C1ql3-mediated synaptic plasticity"**
*作者：Zhang et al. (2019)*
摘要：利用大肠杆菌表达系统制备重组C1ql3蛋白，通过X射线晶体学解析其三维结构，揭示其与神经元表面蛋白相互作用的分子机制，为神经可塑性研究提供结构基础。
3. **"Recombinant C1ql3 protein rescues cognitive deficits in a mouse model of Alzheimer's disease"**
*作者：Li & Wang (2021)*
摘要：研究通过哺乳动物细胞表达重组C1ql3蛋白，证明其在小鼠模型中能逆转突触丢失并改善认知功能，提示C1ql3蛋白在神经退行性疾病治疗中的潜在应用价值。
C1ql3. a member of the C1q/tumor necrosis factor (TNF)-related protein (CTRP) family, is a secreted glycoprotein implicated in diverse biological processes, particularly within the nervous system. The C1q domain, a hallmark of this family, mediates protein-protein interactions and cellular signaling. Recombinant C1ql3 is engineered for experimental studies to explore its structural and functional roles, bypassing challenges associated with endogenous protein purification.
Structurally, C1ql3 features a conserved globular C1q domain at the C-terminus and a variable collagen-like region at the N-terminus, which facilitates multimerization. Recombinant versions are typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, often fused with affinity tags (e.g., His-tag) for simplified purification. Post-translational modifications, such as glycosylation, may require mammalian hosts to preserve native functionality.
Functionally, C1ql3 is enriched in brain regions like the hippocampus and cortex, where it regulates synaptic plasticity by binding to receptors such as BAI3 (ADGRB3), a adhesion G protein-coupled receptor. This interaction influences synapse formation, maintenance, and neurotransmission, linking C1ql3 to cognitive processes. Dysregulation of C1ql3 has been associated with neuropsychiatric disorders (e.g., autism, schizophrenia) and neurodegenerative conditions, highlighting its therapeutic potential.
Recombinant C1ql3 enables *in vitro* studies of ligand-receptor dynamics, structural mapping, and disease modeling. It also serves as a tool for drug screening or gene therapy development targeting C1ql3-associated pathways. Ongoing research aims to clarify its signaling mechanisms and validate its role as a biomarker or intervention target for neurological diseases. Its dual involvement in neural development and pathology underscores the importance of recombinant C1ql3 in bridging basic science and translational applications.
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