| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHF1 |
| Uniprot No | Q96S99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-279aa |
| 氨基酸序列 | MVDHLANTEINSQRIAAVESCFGASGQPLALPGRVLLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLNKRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTAKKSFVVSAASATERQEWISHIEECVRRQLRATGRPPSTEHAAPWIPDKATDICMRCTQTRFSALTRRHHCRKCGFVVCAECSRQRFLLPRLSPKPVRVCSLCYRELAAQQRQEEAEEQGAGSPGQPAHLARPICGASSGDDDDSDEDKEGSRDGDWPSSVEFYASGVAWSAFHS |
| 预测分子量 | 35.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLEKHF1重组蛋白的3篇参考文献示例(注：以下内容为模拟生成，实际文献需通过数据库验证)：
1. **文献名称**：*Structural and functional analysis of PLEKHF1 in endosomal trafficking*
**作者**：Smith A, et al.
**摘要**：本研究通过重组表达人源PLEKHF1蛋白，解析其PH结构域的晶体结构，并揭示其通过结合磷脂酰肌醇参与早期内体膜定位，调控受体酪氨酸激酶的内吞过程。
2. **文献名称**：*PLEKHF1 interacts with G-protein coupled receptors and modulates lysosomal degradation*
**作者**：Chen L, et al.
**摘要**：利用重组PLEKHF1蛋白进行体外结合实验，发现其与特定GPCRs的C末端相互作用，通过抑制溶酶体降解途径延长细胞表面受体信号传导，提示其在细胞信号调控中的新功能。
3. **文献名称**：*Dysregulation of PLEKHF1 in cancer: Recombinant protein-based mechanistic insights*
**作者**：Wang Y, et al.
**摘要**：通过构建重组PLEKHF1突变体，证实其在乳腺癌细胞中异常表达可破坏内体-溶酶体通路，导致EGFR信号持续激活，促进肿瘤细胞增殖和转移。
PLEKHF1 (Pleckstrin Homology and FYVE Domain-Containing Protein 1) is a cytosolic protein encoded by the PLEKHF1 gene, primarily involved in membrane trafficking and intracellular signaling. It contains a pleckstrin homology (PH) domain and a FYVE (Fab1. YOTB, Vac1. EEA1) zinc finger domain, both critical for phosphoinositide binding and subcellular localization. The PH domain facilitates interactions with phosphatidylinositol lipids, enabling membrane association, while the FYVE domain specifically binds phosphatidylinositol 3-phosphate (PI3P), directing the protein to endosomal compartments.
Recombinant PLEKHF1 protein is engineered for in vitro studies to elucidate its molecular functions, including its role in vesicular transport, autophagy, and apoptosis. Studies suggest PLEKHF1 interacts with proteins like AP-1 (Adaptor Protein Complex 1) and TSG101. linking it to endosomal sorting and receptor trafficking pathways. Dysregulation of PLEKHF1 has been implicated in cancer progression and neurological disorders, with altered expression observed in glioblastoma and ovarian cancers, potentially influencing cell proliferation and metastasis.
Producing recombinant PLEKHF1 typically involves heterologous expression in bacterial or mammalian systems, often fused with tags (e.g., His-tag, GST) for purification and detection. This enables biochemical assays, structural studies, and interaction mapping to explore its binding partners and regulatory mechanisms. Its involvement in stress response pathways and crosstalk with autophagy-related proteins like Beclin-1 further highlights its therapeutic relevance. Research on recombinant PLEKHF1 continues to advance understanding of its dual roles in cellular homeostasis and disease pathogenesis.
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