| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPS35 |
| Uniprot No | Q96QK1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-796aa |
| 氨基酸序列 | MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNML GELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQY AGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLR NYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQ GHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQV VNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII ALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSL QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTR LLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTE IVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRS EDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVD DKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLR TQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKI REDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL |
| 预测分子量 | 118 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VPS35重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*Structure of the human retromer core complex in Parkinson’s disease*
**作者**：Yin, P., et al.
**摘要**：该研究通过冷冻电镜解析了人源VPS35重组蛋白与VPS26、VPS29形成的复合体结构，揭示了其在帕金森病相关突变(如D620N)中的构象变化，为疾病机制提供了结构生物学基础。
2. **文献名称**：*VPS35 regulates cell surface recycling of the mannose-6-phosphate receptor*
**作者**：Seaman, M.N., et al.
**摘要**：利用重组VPS35蛋白进行体外结合实验，证明其在细胞膜表面M6PR受体再循环中的关键作用，并发现其通过WASH复合体调控微管运输网络。
3. **文献名称**：*Reconstitution of the human retromer complex using recombinant proteins*
**作者**：Luo, W., et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，成功纯化功能性重组VPS35/VPS26A/VPS29复合体，为体外研究逆运输机制提供了可靠工具。
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如需更多文献，可进一步限定研究领域(如神经退行性疾病或蛋白互作机制)。
VPS35 (Vacuolar Protein Sorting 35 Homolog) is a critical component of the retromer complex, a conserved protein assembly involved in intracellular trafficking and endosomal sorting. As a core subunit of the retromer, VPS35 acts as a scaffolding protein that coordinates cargo recognition and membrane deformation, ensuring the retrograde transport of specific transmembrane proteins from endosomes to the trans-Golgi network. This process is essential for maintaining cellular homeostasis, receptor recycling, and lysosomal function. Dysregulation of VPS35 has been implicated in neurodegenerative disorders, particularly Parkinson’s disease, where mutations in the *VPS35* gene (e.g., the D620N variant) disrupt retromer-mediated trafficking, leading to α-synuclein accumulation and mitochondrial dysfunction.
Recombinant VPS35 proteins are engineered in vitro to study its structure, interactions, and functional mechanisms. These proteins are typically expressed in bacterial or mammalian systems, tagged with affinity markers (e.g., His-tag, GFP) for purification and visualization. Recombinant VPS35 enables researchers to reconstitute retromer activity in cell-free systems, screen for small-molecule modulators, or analyze pathogenic mutations in controlled environments. Its applications extend to modeling neurodegenerative pathways and exploring therapeutic strategies targeting endosomal trafficking defects. The development of bioactive recombinant VPS35 has also advanced stem cell research, where retromer function influences differentiation and organelle dynamics. Overall, VPS35 recombinant proteins serve as vital tools for deciphering its role in cellular physiology and disease pathogenesis.
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