| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Pkdcc |
| Uniprot No | Q504Y2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-493aa |
| 氨基酸序列 | PRPGQSPEPSPAPGPGRRGGRGELARQIRARYEEVQRYSRGGPGPGAGRPERRRLMDLAPGGPGLPRPRPPWARPLSDGAPGWPPAPGPGSPGPGPRLGCAALRNVSGAQYMGSGYTKAVYRVRLPGGAAVALKAVDFSGHDLGSCVREFGVRRGCYRLAAHKLLKEMVLLERLRHPNVLQLYGYCYQDSEDIPDTLTTITELGAPVEMIQLLQTSWEDRFRICLSLGRLLHHLAHSPLGSVTLLDFRPRQFVLVDGELKVTDLDDARVEETPCAGSTDCILEFPARNFTLPCSAQGWCEGMNEKRNLYNAYRFFFTYLLPHSAPPSLRPLLDSIVNATGELAWGVDETLAQLEKVLHLYRSGQYLQNSTASSSTEYQCIPDSTIPQEDYRCWPSYHHGSCLLSVFNLAEAVDVCESHAQCRAFVVTNQTTWTGRQLVFFKTGWSQVVPDPNKTTYVKASG |
| 预测分子量 | 54,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Pkdcc重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Pkdcc Recombinant Protein Regulates Limb Development via Wnt Signaling*
**作者**: Smith J, et al. (2015)
**摘要**: 本研究通过在大肠杆菌中表达并纯化Pkdcc重组蛋白，验证了其在体外激活Wnt/β-catenin信号通路的能力，并证明其通过调控软骨细胞分化影响小鼠胚胎肢体发育。
2. **文献名称**: *Kinase Activity of Recombinant Pkdcc is Essential for Ciliogenesis*
**作者**: Chen L, et al. (2020)
**摘要**: 文章利用HEK293细胞表达重组Pkdcc蛋白，发现其激酶结构域对纤毛形成至关重要，并揭示了Pkdcc通过磷酸化下游靶点调控初级纤毛组装的分子机制。
3. **文献名称**: *Expression and Functional Analysis of Pkdcc in Craniofacial Development*
**作者**: Garcia R, et al. (2018)
**摘要**: 该研究通过重组Pkdcc蛋白的体外功能实验，结合小鼠模型，证明Pkdcc缺失导致颅面骨骼发育异常，可能与BMP信号通路相互作用有关。
4. **文献名称**: *Purification and Characterization of Recombinant Pkdcc for Structural Studies*
**作者**: Lee H, Johnson M (2017)
**摘要**: 作者优化了Pkdcc重组蛋白的昆虫细胞表达系统，获得高纯度蛋白用于结晶实验，初步揭示了其激酶结构域的三维结构及潜在底物结合位点。
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**注意**：以上文献为示例性质，具体研究需参考真实发表的学术论文。建议通过PubMed、Google Scholar等平台以关键词“Pkdcc recombinant protein”或“Pkdcc function”检索最新文献。
Pkdcc (Protein kinase domain-containing protein C), also known as vertebrate lonesome kinase (Vlk), is a secreted tyrosine kinase encoded by the Pkdcc gene. Unlike most kinases, Pkdcc is secreted extracellularly rather than functioning intracellularly, making it a unique member of the protein kinase superfamily. It plays critical roles in embryonic development, particularly in limb formation, chondrocyte differentiation, and skeletal morphogenesis. Studies in knockout mouse models have revealed that Pkdcc deficiency leads to severe limb shortening (mesomelia) and craniofacial abnormalities, highlighting its importance in bone and cartilage development. The protein is thought to regulate fibroblast growth factor (FGF) and sonic hedgehog (SHH) signaling pathways during limb patterning.
Recombinant Pkdcc protein is engineered to study its biochemical properties and therapeutic potential. Produced through heterologous expression systems (e.g., mammalian or insect cells), the recombinant form retains kinase activity and secretion capabilities. Researchers utilize it to investigate phosphorylation targets, receptor interactions, and its role in extracellular signaling cascades. Recent studies also explore its involvement in cancer progression and tissue regeneration. Its unique secretion mechanism and developmental significance make Pkdcc a compelling subject for both basic research and translational applications in skeletal disorders.
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