| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRY2 |
| Uniprot No | Q49AN0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-593aa |
| 氨基酸序列 | MAATVATAAAVAPAPAPGTDSASSVHWFRKGLRLHDNPALLAAVRGARCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVFPRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIELNGQKPPLTYKRFQAIISRMELPKKPVGLVTSQQMESCRAEIQENHDETYGVPSLEELGFPTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFGCLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICIQIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSWESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRYLPKLKAFPSRYIYEPWNAPESIQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQQLSRYRGLCLLASVPSCVEDLSHPVAEPSSSQAGSMSSAGPRPLPSGPASPKRKLEAAEEPPGEELSKRARVAELPTPELPSKDA |
| 预测分子量 | 74.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Spatiotemporal control of cell signalling using a light-switchable protein interaction"**
- **作者**: Kennedy, M.J. 等人 (2010)
- **摘要**: 开发了基于CRY2与CIB1的光诱导二聚化系统，通过蓝光激活蛋白质相互作用，用于动态调控细胞内信号通路。
2. **"Crystal structure of CRY2 PHR domain reveals a conserved photolyase fold with light-dependent conformational changes"**
- **作者**: Nangle, S.N. 等人 (2013)
- **摘要**: 解析了CRY2光解酶同源结构域的晶体结构，揭示了蓝光诱导的构象变化机制，为光遗传学工具优化提供结构基础。
3. **"Optogenetic control of CRISPR/Cas9 gene editing"**
- **作者**: Taslimi, A. 等人 (2014)
- **摘要**: 将CRY2与CIB1融合到CRISPR-Cas9系统，实现蓝光调控的基因编辑，增强时空精度并减少脱靶效应。
4. **"Light-mediated activation of transcriptional function in mammalian cells"**
- **作者**: Liu, H. 等人 (2012)
- **摘要**: 在哺乳动物细胞中利用CRY2光敏特性，设计光控转录调控系统，动态诱导特定基因表达。
CRY2 (Cryptochrome 2) is a light-sensitive protein derived from *Arabidopsis thaliana*, belonging to the cryptochrome family of flavoproteins. Initially identified for its role in regulating circadian rhythms and photomorphogenesis in plants, CRY2 undergoes conformational changes upon exposure to blue light (450-490 nm), enabling its interaction with binding partners like CIB1 (Cryptochrome-Interacting Basic-helix-loop-helix). This light-dependent dimerization property has been harnessed in synthetic biology and optogenetics to develop tools for precise spatiotemporal control of cellular processes.
Recombinant CRY2. engineered for heterologous expression in mammalian and other eukaryotic systems, retains its photosensitivity while being fused to functional domains of interest. Its N-terminal photolyase-related region binds flavin adenine dinucleotide (FAD) as a chromophore, while the C-terminal extension mediates light-induced oligomerization. Upon blue light stimulation, CRY2 undergoes rapid clustering, a reversible process that dissipates in darkness. This dynamic behavior forms the basis for optogenetic platforms such as LightOggle systems, where CRY2 is paired with CIB1 to recruit proteins to specific subcellular locations or trigger signaling cascades.
The versatility of recombinant CRY2 lies in its millisecond-scale activation, low toxicity, and compatibility with live-cell imaging. It has been widely adopted to manipulate protein-protein interactions, membrane remodeling, gene expression, and organelle dynamics with subcellular precision. Recent advancements include photoactivatable CRISPR systems and light-gated ion channels using CRY2-based switches. Challenges remain in minimizing basal activity in dark conditions and optimizing spectral specificity, but ongoing engineering efforts continue to expand its applications in neuroscience, developmental biology, and synthetic biology.
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