| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASIP |
| Uniprot No | P42127 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-132aa |
| 氨基酸序列 | HLPPEEKLRDDRSLRSNSSVNLLDVPSVSIVALNKKSKQIGRKAAEKKRSSKKEASMKKVVRPRTPLSAPCVATRNSCKPPAPACCDPCASCQCRFFRSACSCRVLSLNC |
| 预测分子量 | 19.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASIP重组蛋白的3篇参考文献示例：
1. **"Expression and Purification of Recombinant Agouti Signaling Protein (ASIP) in Escherichia coli"**
- 作者：Smith J. et al.
- 摘要：研究利用大肠杆菌表达系统成功表达并纯化具有生物活性的ASIP重组蛋白，通过His标签亲和层析优化纯化步骤，并验证其体外拮抗MC1R受体的功能。
2. **"Functional Characterization of Recombinant ASIP in Melanocyte Signaling Pathways"**
- 作者：Li Y. et al.
- 摘要：通过昆虫细胞表达系统制备ASIP重组蛋白，分析其对黑色素细胞中cAMP信号通路的抑制作用，揭示ASIP在毛色调控中的分子机制。
3. **"Structural Insights into ASIP-MC1R Interaction Using Recombinant Protein Mutants"**
- 作者：Garcia R. et al.
- 摘要：通过定点突变技术构建ASIP重组蛋白变体，结合X射线晶体学解析ASIP与MC1R的互作结构域，为理解其生理功能提供结构基础。
(注：以上文献为示例，实际引用需根据具体研究查阅真实论文。)
**Background of ASIP Recombinant Protein**
The Agouti Signaling Protein (ASIP) is a endogenous antagonist of melanocortin receptors, primarily targeting the melanocortin 1 receptor (MC1R) in melanocytes. It plays a critical role in regulating pigmentation by competitively inhibiting α-melanocyte-stimulating hormone (α-MSH) binding to MC1R, thereby switching melanin synthesis from eumelanin (dark pigment) to pheomelanin (light pigment). This mechanism is evolutionarily conserved and well-studied in animal models, where ASIP expression patterns determine coat color variations.
Recombinant ASIP protein is produced using biotechnological methods, such as expression in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification. Its production enables detailed study of ASIP’s structure-function relationships, receptor interaction dynamics, and downstream signaling pathways. Researchers utilize recombinant ASIP to investigate its role not only in pigmentation disorders but also in metabolic regulation, as melanocortin receptors are involved in energy homeostasis, appetite control, and insulin sensitivity.
Beyond basic research, ASIP recombinant protein has potential therapeutic applications. Abnormal ASIP expression is linked to conditions like obesity, melanoma, and syndromes associated with pigmentation anomalies (e.g., vitiligo). By modulating MC1R activity, recombinant ASIP could offer insights into drug development for these diseases. However, challenges remain in understanding its tissue-specific effects and ensuring targeted delivery.
Structurally, ASIP contains two conserved domains: an N-terminal domain essential for receptor binding and a C-terminal domain involved in trafficking and secretion. Studies using recombinant variants have clarified how post-translational modifications influence its bioactivity.
Overall, ASIP recombinant protein serves as a vital tool for dissecting melanocortin signaling and exploring novel treatments for pigmentary and metabolic disorders, though further research is needed to translate these findings into clinical practice.
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