| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPRIN1 |
| Uniprot No | Q14444 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-709aa |
| 氨基酸序列 | PSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGTGAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNNLEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAEEYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGMPQMNTQQVN |
| 预测分子量 | 81.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPRIN1重组蛋白的3篇参考文献，涵盖其功能、相互作用及疾病相关性研究：
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1. **文献名称**: *CAPRIN1 promotes colorectal cancer metastasis via binding to ZNF839 and activating the Wnt/β-catenin signaling pathway*
**作者**: Li Y, et al.
**摘要**: 本研究揭示了CAPRIN1重组蛋白通过与转录因子ZNF839结合，激活Wnt/β-catenin信号通路，促进结直肠癌侵袭转移的机制。实验显示CAPRIN1在癌组织中高表达，且与患者预后不良相关。
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2. **文献名称**: *Recombinant CAPRIN1 interacts with G3BP1 and regulates stress granule formation under oxidative stress*
**作者**: Wang X, et al.
**摘要**: 通过体外表达CAPRIN1重组蛋白，发现其与应激颗粒关键蛋白G3BP1直接结合，调控氧化应激下细胞应激颗粒的组装。研究为CAPRIN1在细胞应激反应中的作用提供了分子机制支持。
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3. **文献名称**: *CAPRIN1 is a novel oncogenic driver in triple-negative breast cancer via mTORC1 signaling activation*
**作者**: Chen H, et al.
**摘要**: 利用重组CAPRIN1蛋白进行功能实验，证明其通过激活mTORC1通路促进三阴性乳腺癌细胞增殖和存活。沉默CAPRIN1可抑制肿瘤生长，提示其作为潜在治疗靶点。
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**备注**：以上文献信息为示例性质，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science以“CAPRIN1 recombinant protein”为关键词检索最新研究。
CAPRIN1 (Cell Cycle Associated Protein 1) is a multifunctional RNA-binding protein encoded by the CAPRIN1 gene, playing critical roles in regulating mRNA stability, translation, and cellular stress responses. It is ubiquitously expressed in various tissues and implicated in cell cycle progression, synaptic plasticity, and cancer biology. Structurally, CAPRIN1 contains disordered regions, phosphorylation sites, and RNA-binding motifs that facilitate interactions with target mRNAs and signaling molecules. Its involvement in stress granule formation under cellular stress highlights its role in post-transcriptional gene regulation.
Recombinant CAPRIN1 protein is engineered for experimental studies to dissect its molecular mechanisms. Produced via bacterial (e.g., E. coli) or mammalian expression systems, it retains functional domains necessary for binding RNAs (e.g., c-Myc, Cyclin D2) or proteins (e.g., FMRP, G3BP1). The recombinant form enables investigations into its phosphorylation dynamics, particularly by cyclin-dependent kinases during cell cycle transitions, and its influence on mRNA transport or decay pathways.
Research applications include studying CAPRIN1’s oncogenic roles, as its overexpression correlates with tumor proliferation, metastasis, and therapy resistance in cancers like breast, liver, and glioblastoma. In neuroscience, it is linked to neurodegenerative diseases through interactions with RNA granules in neurons. Recombinant CAPRIN1 also aids in screening therapeutic agents targeting its activity or interactions. However, challenges remain in mimicking its native post-translational modifications, necessitating careful selection of expression systems. Overall, recombinant CAPRIN1 serves as a vital tool for unraveling its pathophysiological contributions and therapeutic potential.
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