| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | Lyz2 |
| Uniprot No | P08905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-148aa |
| 氨基酸序列 | KVYERCEFARTLKRNGMAGYYGVSLADWVCLAQHESNYNTRATNYNRGDQSTDYGIFQINSRYWCNDGKTPRAVNACGINCSALLQDDITAAIQCAKRVVRDPQGIRAWVAWRAHCQNRDLSQYIRNCGV |
| 预测分子量 | 19.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与Lyz2重组蛋白相关的文献摘要示例(文献信息为模拟生成，仅供格式参考)：
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1. **标题**：*Recombinant Lyz2 expression in murine macrophages enhances bacterial clearance*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达小鼠来源的Lyz2重组蛋白，证实其溶菌酶活性显著高于天然提取物，并在巨噬细胞过表达模型中提高对金黄色葡萄球菌的清除效率。
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2. **标题**：*Structural and functional analysis of human LYSC2 recombinant protein in inflammatory bowel disease*
**作者**：Chen L, et al.
**摘要**：利用哺乳动物细胞系统表达人源Lyz2重组蛋白，通过晶体结构解析揭示其催化机制，并在结肠炎小鼠模型中证明其通过调节肠道菌群缓解炎症的作用。
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3. **标题**：*A novel lysozyme-based fusion protein targeting drug-resistant pathogens*
**作者**：Garcia R, et al.
**摘要**：构建Lyz2与噬菌体内溶素的重组融合蛋白，体外实验显示其对耐甲氧西林葡萄球菌(MRSA)的裂解活性提升10倍，为新型抗菌剂开发提供策略。
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注：实际文献需通过PubMed/Google Scholar等平台以关键词"recombinant Lyz2"或"LYZ2 protein engineering"检索获取。
Lyz2 recombinant protein is a engineered form of lysozyme, a naturally occurring antimicrobial enzyme widely studied for its role in innate immunity. Lysozymes (EC 3.2.1.17) are glycoside hydrolases that cleave β-1.4-glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan, leading to cell lysis. The Lyz2 isoform, notably found in avian sources like chicken egg white (HEWL), has become a model system for structural and functional studies due to its stability and well-characterized properties.
Recombinant Lyz2 is produced through heterologous expression in microbial systems (e.g., E. coli or yeast), enabling scalable production with consistent quality. Its compact structure (~14.3 kDa, 129 amino acids) features four disulfide bonds critical for maintaining the active site conformation. The enzyme's α-helix-rich domain and β-sheet-containing substrate-binding cleft have been extensively mapped through X-ray crystallography, providing insights into its catalytic mechanism and pH-dependent activity (optimal at ~pH 5–6).
Biotechnological applications span multiple fields: in pharmaceuticals, Lyz2 serves as a natural preservative and potential antibiotic adjuvant; in food technology, it extends shelf-life through microbial control; in molecular research, it acts as a cell wall disruption agent for bacterial transformation. Recent studies explore engineered variants with enhanced thermostability or modified substrate specificity for tailored industrial uses. Its safety profile and GRAS (Generally Recognized As Safe) status further support commercial adoption. As antibiotic resistance grows, Lyz2 recombinant protein continues to attract interest as an eco-friendly antimicrobial alternative, while remaining a fundamental tool for investigating protein folding, enzyme kinetics, and host-pathogen interactions.
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