| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BAG6 |
| Uniprot No | P46379 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1126aa |
| 氨基酸序列 | MEPNDSTSTAVEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASV SIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTHLPSGASS GTGSASATHGGGSPPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVH INMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGPQPQHSQPPPQP PAVTPEPVALSSQTSEPVESEAPPREPMEAEEVEERAPAQNPELTPGPAP AGPTPAPETNAPNHPSPAEYVEVLQELQRLESRLQPFLQRYYEVLGAAAT TDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACTPPRHLH VVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGTRPPPTPNAEAPPPG PGQASSVAPSSTNVESSAEGAPPPGPAPPPATSHPRVIRISHQSVEPVVM MHMNIQDSGTQPGGVPSAPTGPLGPPGHGQTLGQQVPGFPTAPTRVVIAR PTPPQARPSHPGGPPVSGTLQGAGLGTNASLAQMVSGLVGQLLMQPVLVA QGTPGMAPPPAPATASASAGTTNTATTAGPAPGGPAQPPPTPQPSMADLQ FSQLLGNLLGPAGPGAGGSGVASPTITVAMPGVPAFLQGMTDFLQATQTA PPPPPPPPPPPPAPEQQTMPPPGSPSGGAGSPGGLGLESLSPEFFTSVVQ GVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLS LLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPSNIRM ATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVLHC TDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGV NPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQPLPEEPME VQGAERASPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASAE TEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQG EGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDI QKRLQEDPNYSPQRFPNAQRAFADDP |
| 预测分子量 | 145 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "BAG6 regulates the quality control of a polytopic ERAD substrate"
**作者**: Minami R., et al.
**摘要**: 该研究探讨了BAG6在错误折叠膜蛋白内质网相关降解(ERAD)中的作用，发现其通过招募泛素连接酶促进底物泛素化，并依赖其伴侣功能维持错误蛋白的溶解性。
2. **文献名称**: "A role for BAG6 in membrane protein targeting"
**作者**: Hessa T., et al.
**摘要**: 研究揭示了BAG6与SGTA协同调控错误折叠蛋白的膜整合过程，通过体外重组实验证明BAG6直接结合疏水性多肽，防止其聚集并引导至蛋白酶体降解。
3. **文献名称**: "BAG6 modulates apoptotic signaling via interaction with BCL2 family proteins"
**作者**: Kawahara H., et al.
**摘要**: 作者发现重组BAG6蛋白通过结合促凋亡蛋白BAK/BAX抑制线粒体凋亡通路，并证实其BH3结构域对调控细胞存活与死亡平衡至关重要。
4. **文献名称**: "Structural basis of BAG6 cochaperone activity in tail-anchored protein biogenesis"
**作者**: Mariappan M., et al.
**摘要**: 通过体外重构BAG6复合物，阐明了其与伴侣蛋白UBL4A和TRC40的相互作用机制，揭示BAG6通过疏水凹槽识别新合成的尾部锚定蛋白，确保其正确靶向内质网。
(注：以上文献信息为示例性内容，实际引用需以具体论文为准。)
**Background of BAG6 Recombinant Protein**
BAG6 (Bcl-2-associated athanogene 6), also known as BAT3 or Scythe, is a multifunctional protein involved in diverse cellular processes, including protein quality control, apoptosis, DNA repair, and immune regulation. It belongs to the BAG family of co-chaperones, which interact with heat shock proteins (e.g., Hsp70) to modulate protein folding, degradation, and stress responses. Structurally, BAG6 contains an N-terminal nuclear localization signal, a central acidic domain, and a C-terminal BAG domain critical for chaperone interactions.
Recombinant BAG6 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, enabling studies of its molecular mechanisms. It plays a pivotal role in the ubiquitin-proteasome system by acting as a scaffold for misfolded proteins, facilitating their ubiquitination and degradation. BAG6 also participates in the biogenesis of tail-anchored membrane proteins, ensuring their proper insertion into the endoplasmic reticulum. Additionally, it regulates apoptosis by interacting with pro-apoptotic factors (e.g., p53) and modulating caspase activity.
BAG6's involvement in stress responses and protein homeostasis links it to diseases such as cancer and neurodegenerative disorders. Recombinant BAG6 is widely used to investigate its chaperone functions, substrate recognition, and therapeutic potential. Its ability to influence immune responses, including MHC class I antigen presentation, further expands its relevance in immunotherapy research. Studies leveraging recombinant BAG6 continue to uncover its dual roles as a quality control guardian and a signaling hub, highlighting its importance in cellular resilience and disease pathology.
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