| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAK1 |
| Uniprot No | Q13421 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 296-580aa |
| 氨基酸序列 | EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQG |
| 预测分子量 | 61.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAK1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"CAK1 encodes a CDK-activating kinase in Saccharomyces cerevisiae"*
**作者**：Cross, F.R. et al.
**摘要**：本研究首次在酿酒酵母中鉴定了CAK1基因，证实其编码的蛋白可通过磷酸化CDK(细胞周期蛋白依赖性激酶)激活其活性。通过重组表达CAK1蛋白，证明其在体外能直接磷酸化并激活Cdc28(酵母CDK同源物)，揭示了CAK1在细胞周期调控中的关键作用。
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2. **文献名称**：*"Recombinant CAK1 protein kinase exhibits broad substrate specificity in vitro"*
**作者**：Kaldis, P. & Solomon, M.J.
**摘要**：该研究利用大肠杆菌重组表达并纯化CAK1蛋白，通过体外激酶实验证明其不仅激活CDK，还可磷酸化其他底物(如RNA聚合酶II的CTD结构域)。结果表明CAK1可能具有多功能激酶特性，拓展了对其生物学功能的理解。
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3. **文献名称**：*"Structural and functional analysis of the CAK1-cyclin H complex"*
**作者**：Lolli, G. et al.
**摘要**：通过X射线晶体学解析了重组CAK1蛋白与cyclin H的复合物结构，揭示了二者结合的分子机制。功能实验表明，CAK1的活性受cyclin H的调控，该复合物在CDK7激活中起关键作用，为设计靶向CAK1的抑制剂提供了结构基础。
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**备注**：以上文献为示例性内容，实际检索需通过PubMed或Google Scholar以关键词“CAK1 recombinant protein”或“CAK1 kinase activity”获取具体文献信息。
The CAK1 (Cdk-Activating Kinase 1) recombinant protein is derived from a conserved kinase critical for cell cycle regulation, originally identified in *Saccharomyces cerevisiae* (budding yeast). CAK1 functions as a key activator of cyclin-dependent kinases (CDKs), particularly Cdc28 (the yeast homolog of mammalian CDK1), by phosphorylating a conserved threonine residue in their activation loop. Unlike mammalian CDK-activating kinases (e.g., CDK7), CAK1 operates independently of cyclins and forms a monomeric enzyme, making it a unique model for studying CDK activation mechanisms. Its role in cell cycle progression, transcriptional regulation, and DNA repair has positioned CAK1 as a focal point in understanding eukaryotic cell division and stress responses.
Recombinant CAK1 protein is typically produced using heterologous expression systems, such as *E. coli* or insect cells, enabling high-yield purification of the active kinase. The recombinant form retains its enzymatic activity and structural integrity, allowing researchers to study its biochemical properties, substrate specificity, and interactions in vitro. This has facilitated crystallographic studies, revealing atomic-level details of its ATP-binding pocket and catalytic mechanism.
The protein’s applications span basic research and drug discovery. It is used to screen for kinase inhibitors, dissect signaling pathways, and explore evolutionary divergence among CDK-activating kinases. Additionally, CAK1’s distinct regulatory features offer insights into diseases linked to cell cycle dysregulation, such as cancer. By serving as a simplified yet functional analog of mammalian CDK activators, CAK1 recombinant protein bridges gaps in understanding conserved versus species-specific cell cycle control mechanisms.
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