| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRT |
| Uniprot No | O14522 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-747aa |
| 氨基酸序列 | QSAAGGCSFDEHYSNCGYSVALGTNGFTWEQINTWEKPMLDQAVPTGSFMMVNSSGRASGQKAHLLLPTLKENDTHCIDFHYYFSSRDRSSPGALNVYVKVNGGPQGNPVWNVSGVVTEGWVKAELAISTFWPHFYQVIFESVSLKGHPGYIAVDEVRVLAHPCRKAPHFLRLQNVEVNVGQNATFQCIAGGKWSQHDKLWLQQWNGRDTALMVTRVVNHRRFSATVSVADTAQRSVSKYRCVIRSDGGSGVSNYAELIVKEPPTPIAPPELLAVGATYLWIKPNANSIIGDGPIILKEVEYRTTTGTWAETHIVDSPNYKLWHLDPDVEYEIRVLLTRPGEGGTGPPGPPLTTRTKCADPVHGPQNVEIVDIRARQLTLQWEPFGYAVTRCHSYNLTVQYQYVFNQQQYEAEEVIQTSSHYTLRGLRPFMTIRLRLLLSNPEGRMESEELVVQTEEDVPGAVPLESIQGGPFEEKIYIQWKPPNETNGVITLYEINYKAVGSLDPSADLSSQRGKVFKLRNETHHLFVGLYPGTTYSFTIKASTAKGFGPPVTTRIATKISAPSMPEYDTDTPLNETDTTITVMLKPAQSRGAPVSVYQLVVKEERLQKSRRAADIIECFSVPVSYRNASSLDSLHYFAAELKPANLPVTQPFTVGDNKTYNGYWNPPLSPLKSYSIYFQALSKANGETKINCVRLATKGASTQNSNTVEPEKQVDNTVKM |
| 预测分子量 | 109.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRT重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Mutations in PTPRT promote STAT3 activation in human colorectal carcinomas*
**作者**：Zhang, X., et al.
**摘要**：该研究通过分析结直肠癌样本，发现PTPRT基因的突变导致其磷酸酶活性丧失，无法去磷酸化STAT3.从而增强STAT3信号通路，促进肿瘤发生。研究中使用重组PTPRT蛋白验证了其与STAT3的相互作用及功能影响。
2. **文献名称**：*Expression and purification of the receptor-type protein tyrosine phosphatase PTPRT in insect cells*
**作者**：Wang, Y., et al.
**摘要**：文章描述了利用杆状病毒-昆虫细胞系统高效表达重组人源PTPRT胞内结构域的方法，并通过亲和层析纯化获得高纯度蛋白，为后续酶活性和结构研究提供了基础。
3. **文献名称**：*Structural basis for extracellular domain recognition by the phosphatase PTPRT*
**作者**：Barrera, V., et al.
**摘要**：该研究解析了重组PTPRT蛋白胞外域的晶体结构，揭示了其与细胞粘附分子相互作用的特异性结合界面，阐明了PTPRT在细胞间信号传导中的潜在机制。
(注：以上文献信息为示例，实际引用时需核对具体文献来源及准确性。)
**Background of PTPRT Recombinant Protein**
Protein tyrosine phosphatase receptor type T (PTPRT), also known as PTPrho, is a member of the protein tyrosine phosphatase (PTP) superfamily, which plays critical roles in regulating cellular signaling pathways by dephosphorylating tyrosine residues on target proteins. PTPRT is a transmembrane receptor-type phosphatase characterized by an extracellular fibronectin III domain, a single transmembrane region, and an intracellular catalytic domain. It is broadly expressed in various tissues, including the brain, kidney, and colon, and has been implicated in cell adhesion, growth, and differentiation.
Research highlights PTPRT’s role as a tumor suppressor, particularly in colorectal and lung cancers. Somatic mutations in the *PTPRT* gene are frequently observed in human cancers, often leading to loss of phosphatase activity and dysregulation of signaling pathways, such as STAT3. which promotes tumor progression. Recombinant PTPRT protein, produced via expression systems like mammalian (e.g., HEK293) or insect cells (e.g., Sf9), retains enzymatic activity and structural integrity, enabling functional studies. It is widely used to investigate substrate specificity, inhibition mechanisms, and interactions with signaling molecules.
The recombinant protein also serves as a tool for developing therapeutic strategies targeting PTPRT-deficient cancers. Studies explore its potential in restoring normal phosphatase activity or modulating downstream oncogenic pathways. Despite progress, challenges remain in understanding its full spectrum of substrates and regulatory networks. Overall, PTPRT recombinant protein is a vital resource for dissecting its biological roles and translational applications in cancer and beyond.
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