| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DYNLT3 |
| Uniprot No | P51808 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-116aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEEYHRH CDEVGFNAEE AHNIVKECVD GVLGGEDYNH NNINQWTASI VEQSLTHLVK LGKAYKYIVT CAVVQKSAYG FHTASSCFWD TTSDGTCTVR WENRTMNCIV NVFAIAIVL |
| 预测分子量 | 16 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DYNLT3重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际需根据具体数据库检索)：
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1. **文献名称**: *"Recombinant Human DYNLT3 Expression in E. coli and Its Role in Microtubule Binding"*
**作者**: Smith J, et al.
**摘要**: 本研究成功构建了人源DYNLT3重组蛋白的原核表达系统，通过亲和层析纯化获得高纯度蛋白。体外实验证实重组DYNLT3可与微管蛋白结合，并调控动力蛋白复合物的细胞内运输功能。
2. **文献名称**: *"Structural Analysis of DYNLT3 in Dynein Complex Assembly"*
**作者**: Lee S, et al.
**摘要**: 利用重组DYNLT3蛋白进行X射线晶体学分析，揭示了其与动力蛋白重链相互作用的关键结构域，为理解其在有丝分裂中调控染色体分离的分子机制提供依据。
3. **文献名称**: *"DYNLT3 Recombinant Protein Suppresses Tumor Cell Migration in Glioblastoma Models"*
**作者**: Chen R, et al.
**摘要**: 通过体外表达DYNLT3重组蛋白，发现其能显著抑制胶质母细胞瘤细胞的迁移能力，表明DYNLT3可能通过调节细胞骨架动力学发挥抑癌作用。
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**提示**：实际文献需通过PubMed、Web of Science等平台检索关键词“DYNLT3 recombinant protein”或“DYNLT3 expression”。建议结合研究领域(如癌症、神经生物学)进一步筛选。
DYNLT3. also known as dynein light chain Tctex-type 3. is a member of the dynein light chain family that plays a role in intracellular transport and microtubule-based motility. It is a component of the cytoplasmic dynein complex, a multi-subunit motor protein responsible for retrograde transport of vesicles, organelles, and macromolecules along microtubules. DYNLT3 interacts with the heavy chain of dynein and other regulatory subunits, contributing to cargo binding and regulation of motor activity. Structurally, it contains a conserved Tctex1 domain, which facilitates protein-protein interactions essential for its function.
Recombinant DYNLT3 protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity tags (e.g., His-tag) and chromatography. Its recombinant form retains the native structure and biological activity, making it a valuable tool for studying dynein-mediated processes. Research has linked DYNLT3 to diverse cellular functions, including mitotic spindle organization, ciliogenesis, and neuronal trafficking. Dysregulation of DYNLT3 has been implicated in developmental disorders and cancers, with studies suggesting its role in tumor cell proliferation, migration, and chemoresistance.
In biomedical research, recombinant DYNLT3 is used to investigate molecular mechanisms of dynein-associated pathways, protein interaction networks, and cargo specificity. It also serves as an antigen for antibody development or a control in diagnostic assays. Recent studies explore its potential as a therapeutic target, particularly in cancers where dynein dysfunction alters cell division or metastasis. However, its precise regulatory roles and tissue-specific functions remain under active investigation, highlighting the need for further structural and functional characterization.
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