| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRDM16 |
| Uniprot No | Q9HAZ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1276aa |
| 氨基酸序列 | MRSKARARKLAKSDGDVVNNMYEPNRDLLASHSAEDEAEDSAMSPIPVGPPSPFPTSEDFTPKEGSPYEAPVYIPEDIPIPADFELRESSIPGAGLGVWAKRKMEAGERLGPCVVVPRAAAKETDFGWEQILTDVEVSPQEGCITKISEDLGSEKFCVDANQAGAGSWLKYIRVACSCDDQNLTMCQISEQIYYKVIKDIEPGEELLVHVKEGVYPLGTVPPGLDEEPTFRCDECDELFQSKLDLRRHKKYTCGSVGAALYEGLAEELKPEGLGGGSGQAHECKDCERMFPNKYSLEQHMVIHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKRFECENCVKVFTDPSNLQRHIRSQHVGARAHACPDCGKTFATSSGLKQHKHIHSTVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHYTPGGIFAPGLPLTPSPMMDKAKPSPSLNHASLGFNEYFPSRPHPGSLPFSTAPPTFPALTPGFPGIFPPSLYPRPPLLPPTSLLKSPLNHTQDAKLPSPLGNPALPLVSAVSNSSQGTTAAAGPEEKFESRLEDSCVEKLKTRSSDMSDGSDFEDVNTTTGTDLDTTTGTGSDLDSDVDSDPDKDKGKGKSAEGQPKFGGGLAPPGAPNSVAEVPVFYSQHSFFPPPDEQLLTATGAAGDSIKAIASIAEKYFGPGFMGMQEKKLGSLPYHSAFPFQFLPNFPHSLYPFTDRALAHNLLVKAEPKSPRDALKVGGPSAECPFDLTTKPKDVKPILPMPKGPSAPASGEEQPLDLSIGSRARASQNGGGREPRKNHVYGERKLGAGEGLPQVCPARMPQQPPLHYAKPSPFFMDPIYSRVEKRKVTDPVGALKEKYLRPSPLLFHPQMSAIETMTEKLESFAAMKADSGSSLQPLPHHPFNFRSPPPTLSDPILRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCNRCFGQQTNLDRHLKKHEHENAPVSQHPGVLTNHLGTSASSPTSESDNHALLDEKEDSYFSEIRNFIANSEMNQASTRTEKRADMQIVDGSAQCPGLASEKQEDVEEEDDDDLEEDDEDSLAGKSQDDTVSPAPEPQAAYEDEEDEEPAASLAVGFDHTRRCAEDHEGGLLALEPMPTFGKGLDLRRAAEEAFEVKDVLNSTLDSEALKHTLCRQAKNQAYAMMLSLSEDTPLHTPSQGSLDAWLKVTGATSESGAFHPINHL |
| 预测分子量 | 140 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRDM16重组蛋白的3-4篇文献摘要概览：
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1. **文献名称**：*PRDM16 controls a brown fat/skeletal muscle switch*
**作者**：Kajimura S. 等
**摘要**：该研究揭示了PRDM16作为棕色脂肪细胞分化的关键调控因子，通过重组蛋白实验证明其与转录复合物相互作用，促进棕色脂肪生成并抑制肌肉细胞分化通路。
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2. **文献名称**：*Prdm16 is required for the maintenance of long-term hematopoietic stem cells*
**作者**：Chuikov S. 等
**摘要**：文章发现PRDM16通过调控下游靶基因(如Notch通路)维持造血干细胞的自我更新能力，重组蛋白分析显示其缺失导致白血病前状态的异常分化。
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3. **文献名称**：*PRDM16 interacts with the mediator complex to regulate adipogenesis*
**作者**：Harms M.J. 等
**摘要**：研究利用重组PRDM16蛋白发现其通过结合中介体复合物(Mediator Complex)激活脂肪生成相关基因，并抑制白色脂肪细胞的炎症信号，影响代谢平衡。
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4. **文献名称**：*PRDM16 suppresses MLL1r leukemia via intrinsic histone methyltransferase activity*
**作者**：Hirata H. 等
**摘要**：通过重组PRDM16的功能实验，证明其通过组蛋白甲基转移酶活性抑制混合系白血病(MLL1r)的进展，为白血病治疗提供潜在靶点。
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这些文献涵盖了PRDM16在脂肪分化、造血调控、代谢疾病及癌症中的作用，重点围绕其分子机制与重组蛋白的功能验证。
PRDM16 (PR Domain-Containing Protein 16) is a transcriptional regulator that plays critical roles in cell fate determination, tissue development, and metabolic homeostasis. It belongs to the PRDM protein family characterized by a conserved PR (PRDI-BF1 and RIZ homology) domain, which often exhibits histone methyltransferase activity. PRDM16 contains multiple functional domains, including zinc finger motifs that mediate DNA binding and protein-protein interactions. It is best known for its dual role in regulating adipocyte differentiation and maintaining brown/beige adipose tissue thermogenesis. By interacting with transcriptional coactivators (e.g., PPARγ, C/EBPβ) or corepressors, PRDM16 modulates the balance between energy-storing white adipocytes and energy-dissipating thermogenic adipocytes.
In hematopoiesis, PRDM16 is essential for maintaining hematopoietic stem cell quiescence and myeloid lineage specification. Chromosomal translocations involving PRDM16 are associated with acute myeloid leukemia (AML), where its aberrant fusion proteins disrupt normal differentiation. Beyond metabolic and hematopoietic systems, PRDM16 influences cardiac development, neural crest cell migration, and mitochondrial biogenesis.
Recombinant PRDM16 proteins are engineered to study its molecular mechanisms and therapeutic potential. These proteins are typically produced in expression systems (e.g., E. coli, mammalian cells) with affinity tags (e.g., His-tag, GST) for purification. Researchers use recombinant PRDM16 to investigate its DNA-binding specificity, enzymatic activity, and interactions with partner proteins in vitro. Mutant variants help map functional domains or model disease-associated mutations. In drug discovery, recombinant PRDM16 serves as a tool for screening compounds that modulate its activity, particularly for obesity-related metabolic disorders or hematologic malignancies. Current challenges include maintaining the protein's structural integrity due to its large size (~200 kDa) and complex domain architecture.
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