| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX23 |
| Uniprot No | Q9BUQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-820aa |
| 氨基酸序列 | MAGELADKKDRDASPSKEERKRSRTPDRERDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSAERERRHKERERDKERDRNKKDRDRDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKDEEDEHGDKKPKAQPLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQRMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA |
| 预测分子量 | 95,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural insights into the RNA-dependent cleavage and ligation activities of DDX23 in spliceosome assembly"**
*Author: Zhang Y, et al.*
摘要：该研究通过重组表达DDX23蛋白，结合冷冻电镜技术解析了其在剪接体组装中的构象变化，揭示了DDX23依赖RNA结合调控前体mRNA剪接的分子机制。
2. **"Functional characterization of recombinant DDX23 in viral RNA sensing and innate immunity"**
*Author: Lee S, et al.*
摘要：通过大肠杆菌系统表达并纯化DDX23重组蛋白，发现其通过识别病毒RNA激活RIG-I信号通路，证明DDX23在抗病毒天然免疫反应中的关键作用。
3. **"DDX23 interacts with CWC23 to promote pre-mRNA splicing efficiency"**
*Author: Wang L, et al.*
摘要：利用重组DDX23和CWC23蛋白进行体外结合实验，证明两者形成复合物并协同增强剪接体对分支位点的识别，揭示了剪接因子间的功能关联。
4. **"Recombinant DDX23 overexpression drives glioma progression via mTOR pathway activation"**
*Author: Chen X, et al.*
摘要：在胶质瘤细胞中过表达重组DDX23蛋白，发现其通过结合mTOR mRNA促进翻译，激活促癌信号通路，为靶向DDX23的肿瘤治疗提供依据。
DDX23. also known as DEAD-box helicase 23. is a member of the DEAD-box RNA helicase family characterized by the conserved Asp-Glu-Ala-Asp (DEAD) motif. These ATP-dependent enzymes play pivotal roles in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and RNA transport. DDX23 specifically functions in the spliceosome assembly during pre-mRNA processing, where it interacts with components of the U5 snRNP complex and facilitates the recognition of splice sites. Its helicase activity helps unwind RNA secondary structures, enabling proper spliceosome remodeling.
Recombinant DDX23 protein is produced through heterologous expression systems (e.g., E. coli, insect, or mammalian cells) using genetic engineering techniques to enable controlled study of its biochemical properties. The recombinant form typically retains conserved domains critical for function: two RecA-like domains responsible for ATP hydrolysis and RNA binding, and a C-terminal domain involved in protein-protein interactions. Researchers utilize purified recombinant DDX23 to investigate its enzymatic kinetics, RNA-binding specificity, and structural features through methods like cryo-EM or X-ray crystallography.
Emerging studies link DDX23 dysregulation to diseases, including cancer and viral infections. It interacts with viral RNA in host defense mechanisms, making it a potential antiviral target. Recombinant DDX23 serves as a tool for screening small-molecule inhibitors or studying virus-host interactions. Additionally, its role in alternative splicing regulation has implications in neurodegenerative disorders and oncogenesis, where aberrant splicing contributes to disease progression. The development of recombinant DDX23 thus bridges molecular biology insights with therapeutic exploration.
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