| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SIAT1 |
| Uniprot No | P15907 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-406aa |
| 氨基酸序列 | MIHTNLKKKFSCCVLVFLLFAVICVWKEKKKGSYYDSFKLQTKEFQVLKSLGKLAMGSDSQSVSSSSTQDPHRGRQTLGSLRGLAKAKPEASFQVWNKDSSSKNLIPRLQKIWKNYLSMNKYKVSYKGPGPGIKFSAEALRCHLRDHVNVSMVEVTDFPFNTSEWEGYLPKESIRTKAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNGAPTANFQQDVGTKTTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQNPDYNFFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEKNLVKHLNQGTDEDIYLLGKATLPGFRTIHC |
| 预测分子量 | 52.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SIAT1(唾液酸转移酶1)重组蛋白研究的3篇参考文献示例(内容为模拟概括，仅供参考)：
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1. **文献名称**: "Cloning and functional characterization of human SIAT1 (ST3Gal-I) in recombinant mammalian expression systems"
**作者**: Smith A, et al.
**摘要**: 研究报道了通过克隆人源SIAT1基因并在HEK293细胞中表达重组蛋白，验证其催化唾液酸转移至Galβ1-3GalNAc糖链的活性，为研究肿瘤相关糖基化机制提供工具。
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2. **文献名称**: "Recombinant SIAT1 enzyme production in E. coli and its application in synthesizing sialylated glycoconjugates"
**作者**: Zhang L, et al.
**摘要**: 开发了在大肠杆菌中高效表达可溶性SIAT1重组蛋白的优化方法，并证明其可用于体外合成唾液酸化寡糖，为工业化制备糖类药物奠定基础。
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3. **文献名称**: "Role of SIAT1-mediated sialylation in cancer cell adhesion: Insights from recombinant protein studies"
**作者**: Tanaka K, et al.
**摘要**: 利用重组SIAT1蛋白处理结肠癌细胞，发现其通过增强细胞表面sLeX抗原表达促进肿瘤转移，揭示了SIAT1在癌症进展中的潜在调控机制。
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**注**：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science等平台以关键词“SIAT1 recombinant”或“ST3Gal-I expression”检索最新文献。
SIAT1 (sialyltransferase 1), also known as ST3Gal-I, is a key enzyme in the biosynthesis of sialylated glycoconjugates. It belongs to the sialyltransferase family, which catalyzes the transfer of sialic acid (a nine-carbon sugar) from donor molecules (CMP-sialic acid) to specific acceptor substrates, such as glycoproteins or glycolipids. SIAT1 specifically mediates the addition of α2.3-linked sialic acid to galactose residues, playing a critical role in post-translational modification of cell surface molecules. This modification is essential for cellular recognition, immune response modulation, and pathogen-host interactions.
Recombinant SIAT1 protein is produced using biotechnological methods, often through expression in mammalian or insect cell systems to ensure proper glycosylation and enzymatic activity. The purified protein retains its functional capacity to synthesize sialoglycans, making it a valuable tool for studying glycan biosynthesis, cell signaling, and disease mechanisms. Aberrant sialylation mediated by SIAT1 has been implicated in cancer progression (e.g., enhancing tumor cell invasiveness and immune evasion), neurological disorders, and viral infections (e.g., influenza virus binding).
Researchers utilize recombinant SIAT1 to investigate its role in pathological processes, develop glycoengineering strategies for therapeutic proteins, or screen inhibitors targeting sialylation-related diseases. Its application extends to structural studies for drug design and biomanufacturing of sialylated biologics, such as antibodies with optimized pharmacokinetic properties. The protein’s versatility underscores its importance in both basic research and biopharmaceutical innovation.
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