| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUM1 |
| Uniprot No | Q2TAK8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-710aa |
| 氨基酸序列 | MADAKYVLCR WEKRLWPAKV LARTATSTKN KRRKEYFLAV QILSLEEKIK VKSTEVEILE KSQIEAIASS LASQNEVPAA PLEELAYRRS LRVALDVLSE GSIWSQESSA GTGRADRSLR GKPMEHVSSP CDSNSSSLPR GDVLGSSRPH RRRPCVQQSL SSSFTCEKDP ECKVDHKKGL RKSENPRGPL VLPAGGGAQD ESGSRIHHKN WTLASKRGGN SAQKASLCLN GSSLSEDDTE RDMGSKGGSW AAPSLPSGVR EDDPCANAEG HDPGLPLGSL TAPPAPEPSA CSEPGECPAK KRPRLDGSQR PPAVQLEPMA AGAAPSPGPG PGPRESVTPR STARLGPPPS HASADATRCL PCPDSQKLEK ECQSSEESMG SNSMRSILEE DEEDEEPPRV LLYHEPRSFE VGMLVWHKHK KYPFWPAVVK SVRQRDKKAS VLYIEGHMNP KMKGFTVSLK SLKHFDCKEK QTLLNQARED FNQDIGWCVS LITDYRVRLG CGSFAGSFLE YYAADISYPV RKSIQQDVLG TKLPQLSKGS PEEPVVGCPL GQRQPCRKML PDRSRAARDR ANQKLVEYIV KAKGAESHLR AILKSRKPSR WLQTFLSSSQ YVTCVETYLE DEGQLDLVVK YLQGVYQEVG AKVLQRTNGD RIRFILDVLL PEAIICAISA VDEVDYKTAE EKYIKGPSLS YREKEIFDNQ LLEERNRRRR |
| 预测分子量 | 78,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUM1(IRF4)重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要内容概括：
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1. **文献名称**：*"Expression and functional characterization of recombinant MUM1/IRF4 in B-cell malignancies"*
**作者**：Tsuboi K, et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功制备重组MUM1蛋白，并验证其在体外对B细胞淋巴瘤细胞系的转录调控功能，揭示其通过结合特定DNA序列促进肿瘤细胞存活。
2. **文献名称**：*"Structural insights into the DNA-binding activity of IRF4/MUM1 in multiple myeloma"*
**作者**：García-Sanz R, et al.
**摘要**：通过X射线晶体学解析重组人源MUM1蛋白的DNA结合域结构，阐明其与多发性骨髓瘤相关基因启动子的相互作用机制，为靶向治疗提供分子基础。
3. **文献名称**：*"Recombinant IRF4/MUM1 protein modulates Th17 differentiation in autoimmune disease models"*
**作者**：Li H, et al.
**摘要**：利用昆虫细胞表达系统生产功能性重组MUM1蛋白，发现其通过调控IL-17等细胞因子表达参与Th17细胞分化，提示其在自身免疫疾病中的潜在治疗价值。
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(注：以上文献信息为示例，实际引用需以真实发表的论文为准。)
MUM1 (Multiple Myeloma Oncogene 1), also known as IRF4 (Interferon Regulatory Factor 4), is a transcription factor belonging to the interferon regulatory factor family. It plays critical roles in immune regulation, lymphocyte differentiation, and oncogenesis. Initially identified through its involvement in chromosomal translocations in multiple myeloma, MUM1/IRF4 is encoded by the *IRF4* gene on human chromosome 6p25.3. It regulates gene expression by binding to interferon-sensitive response elements (ISREs) and participates in signaling pathways mediated by cytokines, antigen receptors, and Toll-like receptors. In immune cells, MUM1/IRF4 is essential for plasma cell differentiation, T-cell activation, and the development of regulatory T cells, making it pivotal in both adaptive immunity and immune tolerance.
Dysregulation of MUM1/IRF4 is linked to several malignancies. Overexpression or mutations are observed in lymphoid cancers, including multiple myeloma, diffuse large B-cell lymphoma, and peripheral T-cell lymphoma. Its oncogenic potential stems from its ability to promote cell proliferation, inhibit apoptosis, and alter immune surveillance. In research, recombinant MUM1 protein is generated using expression systems (e.g., *E. coli* or mammalian cells) for functional studies, structural analysis, and drug screening. Purified recombinant MUM1 is utilized to investigate its DNA-binding properties, interactions with co-regulators, and role in disease mechanisms. Additionally, it serves as a tool for developing targeted therapies, such as small-molecule inhibitors or immunotherapies aimed at disrupting its oncogenic activity. As a biomarker, MUM1/IRF4 expression aids in diagnosing specific lymphoma subtypes. Ongoing research continues to explore its dual role in immunity and cancer, highlighting its therapeutic and diagnostic potential.
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