| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDHa2 |
| Uniprot No | P29803 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-388aa |
| 氨基酸序列 | SSN DATFEIKKCD LYLLEEGPPV TTVLTRAEGL KYYRMMLTVR RMELKADQLY KQKFIRGFCH LCDGQEACCV GLEAGINPSD HVITSYRAHG VCYTRGLSVR SILAELTGRR GGCAKGKGGS MHMYTKNFYG GNGIVGAQGP LGAGIALACK YKGNDEICLT LYGDGAANQG QIAEAFNMAA LWKLPCVFIC ENNLYGMGTS TERAAASPDY YKRGNFIPGL KVDGMDVLCV REATKFAANY CRSGKGPILM ELQTYRYHGH SMSDPGVSYR TREEIQEVRS KRDPIIILQD RMVNSKLATV EELKEIGAEV RKEIDDAAQF ATTDPEPHLE ELGHHIYSSD SSFEVRGANP WIKFKSVS |
| 预测分子量 | 42,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDHa2重组蛋白的3篇参考文献示例，涵盖表达、功能及疾病关联研究：
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1. **文献名称**：*"Expression and Functional Analysis of Recombinant PDHa2 in a Prokaryotic System"*
**作者**：Chen, L., et al.
**摘要**：本研究成功在大肠杆菌中表达了重组PDHa2蛋白，优化了诱导条件以提高可溶性表达。通过镍柱亲和层析纯化获得高纯度蛋白，并证实其与PDH复合体其他亚基的相互作用，为后续酶活性和结构研究奠定基础。
2. **文献名称**：*"PDHa2 Deficiency Disrupts Metabolic Homeostasis via Impaired Mitochondrial Function in Hepatocytes"*
**作者**：Wang, Y., et al.
**摘要**：利用重组PDHa2蛋白进行体外功能分析，发现其缺失导致肝细胞线粒体丙酮酸氧化能力下降，引发脂质蓄积和胰岛素抵抗，提示PDHa2在代谢综合征中的潜在调控作用。
3. **文献名称**：*"Structural Characterization of Phosphorylation-Dependent PDHa2 Regulation Using Recombinant Protein"*
**作者**：Kim, S., et al.
**摘要**：通过重组表达人源PDHa2并结合质谱分析，揭示了其关键磷酸化位点(Ser293)对酶活性的抑制作用，解析了磷酸化修饰调控PDH复合体功能的分子机制。
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以上文献示例聚焦PDHa2重组蛋白的表达策略、代谢功能及结构调控，反映了其在基础研究与疾病机制中的重要性。如需真实文献，建议通过PubMed或Web of Science以关键词“PDHA2 recombinant”“pyruvate dehydrogenase alpha subunit”进一步检索。
PDHa2 (Pyruvate Dehydrogenase Alpha 2) is a critical subunit of the pyruvate dehydrogenase (PDH) complex, a mitochondrial multi-enzyme system essential for cellular energy metabolism. The PDH complex catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA, linking glycolysis to the tricarboxylic acid (TCA) cycle. This reaction is a key regulatory step in glucose homeostasis, ensuring efficient ATP production under aerobic conditions. The PDH complex comprises three core enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), and dihydrolipoamide dehydrogenase (E3). The E1 component, a heterotetramer of two α (PDHA) and two β (PDHB) subunits, is responsible for the rate-limiting decarboxylation step.
PDHa2. encoded by the PDHA2 gene, is a tissue-specific isoform of the PDH E1α subunit. Unlike the ubiquitously expressed PDHA1. PDHa2 is predominantly found in testis and skeletal muscle, suggesting specialized roles in these tissues. Mutations in PDHA1 are linked to PDH deficiency, a metabolic disorder causing lactic acidosis and neurological impairments. However, PDHa2's physiological relevance remains less characterized, though its expression patterns imply potential roles in energy-demanding processes like spermatogenesis or muscle contraction.
Recombinant PDHa2 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its purification enables investigations into isoform-specific enzymatic properties, regulatory mechanisms (e.g., phosphorylation by PDK kinases), and interactions within the PDH complex. Researchers also utilize recombinant PDHa2 to model tissue-specific metabolic adaptations, screen therapeutic agents for PDH-related disorders, or develop diagnostic tools. Structural and biochemical analyses of this isoform could unveil novel regulatory pathways, offering insights into metabolic diseases or energy dysregulation in cancers.
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