| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUPT5H |
| Uniprot No | O00267 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1087aa |
| 氨基酸序列 | MSDSEDSNFSEEEDSERSSDGEEAEVDEERRSAAGSEKEEEPEDEEEEEEEEEYDEEEEEEDDDRPPKKPRHGGFILDEADVDDEYEDEDQWEDGAEDILEKEEIEASNIDNVVLDEDRSGARRLQNLWRDQREEELGEYYMKKYAKSSVGETVYGGSDELSDDITQQQLLPGVKDPNLWTVKCKIGEERATAISLMRKFIAYQFTDTPLQIKSVVAPEHVKGYIYVEAYKQTHVKQAIEGVGNLRLGYWNQQMVPIKEMTDVLKVVKEVANLKPKSWVRLKRGIYKDDIAQVDYVEPSQNTISLKMIPRIDYDRIKARMSLKDWFAKRKKFKRPPQRLFDAEKIRSLGGDVASDGDFLIFEGNRYSRKGFLFKSFAMSAVITEGVKPTLSELEKFEDQPEGIDLEVVTESTGKEREHNFQPGDNVEVCEGELINLQGKILSVDGNKITIMPKHEDLKDMLEFPAQELRKYFKMGDHVKVIAGRFEGDTGLIVRVEENFVILFSDLTMHELKVLPRDLQLCSETASGVDVGGQHEWGELVQLDPQTVGVIVRLERETFQVLNMYGKVVTVRHQAVTRKKDNRFAVALDSEQNNIHVKDIVKVIDGPHSGREGEIRHLFRSFAFLHCKKLVENGGMFVCKTRHLVLAGGSKPRDVTNFTVGGFAPMSPRISSPMHPSAGGQRGGFGSPGGGSGGMSRGRGRRDNELIGQTVRISQGPYKGYIGVVKDATESTARVELHSTCQTISVDRQRLTTVGSRRPGGMTSTYGRTPMYGSQTPMYGSGSRTPMYGSQTPLQDGSRTPHYGSQTPLHDGSRTPAQSGAWDPNNPNTPSRAEEEYEYAFDDEPTPSPQAYGGTPNPQTPGYPDPSSPQVNPQYNPQTPGTPAMYNTDQFSPYAAPSPQGSYQPSPSPQSYHQVAPSPAGYQNTHSPASYHPTPSPMAYQASPSPSPVGYSPMTPGAPSPGGYNPHTPGSGIEQNSSDWVTTDIQVKVRDTYLDTQVVGQTGVIRSVTGGMCSVYLKDSEKVVSISSEHLEPITPTKNNKVKVILGEDREATGVLLSIDGEDGIVRMDLDEQLKILNLRFLGKLLEA |
| 预测分子量 | 121 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUPT5H重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: "Structural and functional analysis of recombinant SUPT5H in RNA polymerase II pausing"
**作者**: Zhang L, et al.
**摘要**: 研究利用大肠杆菌表达系统纯化重组SUPT5H蛋白，结合冷冻电镜技术解析其与RNA聚合酶II复合物的相互作用，揭示SUPT5H在转录暂停调控中的关键构象变化。
2. **标题**: "SUPT5H recombinant protein enhances HIV-1 transcription by modulating Tat-mediated elongation"
**作者**: Patel R, Smith T.
**摘要**: 通过昆虫细胞系统表达重组SUPT5H，发现其与HIV Tat蛋白协同促进病毒转录延伸，为靶向SUPT5H的抗病毒治疗提供理论依据。
3. **标题**: "Expression optimization and biochemical characterization of human SUPT5H in a eukaryotic system"
**作者**: Kim H, et al.
**摘要**: 优化哺乳动物细胞中SUPT5H重组蛋白的表达条件，并验证其与DSIF复合物其他组分(如SUPT4H)的结合活性，为体外转录研究提供高纯度蛋白。
4. **标题**: "SUPT5H mutations disrupt transcriptional regulation in cancer: Insights from recombinant mutant protein assays"
**作者**: Gonzalez M, et al.
**摘要**: 构建SUPT5H癌症相关突变体重组蛋白，通过体外实验证明特定突变导致其与染色质修饰因子的相互作用缺陷，进而促进肿瘤异常基因表达。
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注：以上文献为模拟生成，实际引用请通过学术数据库(如PubMed、Web of Science)检索真实研究。
The SUPT5H gene (Suppressor of Ty 5 Homolog) encodes a conserved regulatory protein, SPT5. which functions as a critical component of the transcription elongation machinery in eukaryotes. As part of the SPT4/SPT5 heterodimer complex (DSIF), it interacts directly with RNA polymerase II (Pol II) to modulate transcriptional elongation, pausing, and termination. SPT5 contains multiple structural domains, including a NusG N-terminal domain and Kyprides-Ouzounis-Woese (KOW) repeats, which facilitate its interactions with Pol II, nucleic acids, and other elongation factors like P-TEFb. These interactions are essential for coordinating transcription with co-transcriptional processes such as mRNA capping, splicing, and chromatin remodeling.
Recombinant SUPT5H protein is typically produced in bacterial or mammalian expression systems, often fused with affinity tags (e.g., His-tag) for purification. Its production enables biochemical studies to dissect mechanisms of transcription regulation, including how post-translational modifications (e.g., phosphorylation) or disease-associated mutations alter its function. Dysregulation of SUPT5H has been implicated in cancers, neurodevelopmental disorders, and viral pathogenesis (e.g., HIV), as many viruses hijack host transcription machinery. Additionally, SPT5’s role in maintaining transcription-replication conflict resolution highlights its potential as a therapeutic target. Recombinant forms are widely used in in vitro transcription assays, structural studies (cryo-EM), and drug screening platforms aiming to restore transcriptional fidelity in diseases. Despite its well-characterized role in elongation, ongoing research explores its involvement in non-coding RNA biogenesis and epigenetic regulation, underscoring its multifaceted importance in gene expression control.
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