| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C17orf75 |
| Uniprot No | Q9HAS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MLPSLQESMD GDEKELESSE EGGSAEERRL EPPSSSHYCL YSYRGSRLAQ QRGDSEDGSP SGTNAETPSG DDFSLSLADT NLPSEVEPEL RSFIAKRLSR GAVFEGLGNV ASVELKIPGY RVGCYYCLFQ NEKLLPETVT IDSERNPSEY VVCFLGGSEK GLELFRLELD KYIQGLKNNM NCEARGLESH IKSYLSSWFE DVVCPIQRVV LLFQEKLTFL LHAALSYTPV EVKESDEKTK RDINRFLSVA SLQGLIHEGT MTSLCMAMTE EQHKSVVIDC SSSQPQFCNA GSNRFCEDWM QAFLNGAKGG NPFLFRQVLE NFKLKAIQDT NNLKRFIRQA EMNHYALFKC YMFLKNCGSG DILLKIVKVE HEEMPEAKNV IAVLEEFMKE ALDQSF |
| 预测分子量 | 44,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C17orf75重组蛋白的虚构参考文献示例(实际文献需通过学术数据库验证)：
---
1. **文献名称**: *Functional characterization of recombinant C17orf75 protein in cellular apoptosis*
**作者**: Wang, X. et al.
**摘要**: 本研究通过表达纯化C17orf75重组蛋白，发现其通过调控Bcl-2家族蛋白抑制线粒体途径的细胞凋亡，提示其在癌症耐药性中的潜在作用。
2. **文献名称**: *C17orf75 recombinant protein promotes oxidative stress resistance via NRF2 pathway*
**作者**: Kim, S. & Patel, R.
**摘要**: 体外实验表明，C17orf75重组蛋白通过激活NRF2信号通路增强细胞对氧化应激的耐受性，可能成为抗氧化治疗的分子靶点。
3. **文献名称**: *Structural and biophysical analysis of human C17orf75 recombinant protein*
**作者**: González, L. et al.
**摘要**: 利用X射线晶体学解析C17orf76重组蛋白的三维结构，揭示其与DNA结合域的独特构象，为功能机制研究提供结构基础。
---
**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索真实文献(截至2023年，C17orf75相关研究较少，建议结合基因别名或最新功能研究扩大检索范围)。
**Background of C17orf75 Recombinant Protein**
C17orf75. also known as chromosome 17 open reading frame 75. is a protein-coding gene located on human chromosome 17. The biological functions of the encoded protein remain partially characterized, though emerging studies suggest its potential involvement in cellular processes such as cell proliferation, intracellular signaling, and stress response. Structural predictions indicate that C17orf75 may contain conserved domains associated with protein-protein interactions or enzymatic activity, though experimental validation is ongoing.
Recombinant C17orf75 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cell lines) to produce purified protein for functional and biochemical studies. Its recombinant form enables researchers to investigate its molecular interactions, post-translational modifications, and regulatory roles in pathways linked to diseases. For instance, C17orf75 has been indirectly associated with cancer progression due to its differential expression in tumor tissues and potential crosstalk with oncogenic signaling networks. Additionally, its expression in the nervous system hints at roles in neurological disorders, though mechanistic insights remain limited.
The production of recombinant C17orf75 protein is critical for generating antibodies, structural studies (e.g., crystallography), and high-throughput screening for drug discovery. Challenges include optimizing protein solubility and stability, given its unknown native conformation. Current research focuses on elucidating its interactome, subcellular localization, and relevance to disease biomarkers or therapeutic targets. Further studies are needed to define its precise physiological and pathological contributions, highlighting C17orf75 as a protein of growing interest in molecular and translational research.
×
